The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

• MgCl₂ and dNTPs are included at optimised concentrations
• Master Mix is stable after multiple freeze thaws, reducing wastage and increasing batch-to-batch reliability
• Start Taq polymerase enzyme requires only three minutes for activation
• The dye is non-toxic and can be added at saturating concentrations - insuring minimal inhibition while preserving high sensitivity
• Master Mix can be used with the AriaMx Real-Time PCR System or any HRM-capable thermal cycler

The Master Mix combines a mutant Fast-Start Taq polymerase, optimised concentrations of MgCl₂ and dNTPs, and EvaGreen, release-on-demand dye to enable researchers to mix-and-go faster and with total confidence - even for the most difficult genotypes.

The optimised MgCl₂ and dNTP concentrations reduce assay preparation time, while low surfactant levels minimise bubble trouble during pipetting. In addition, the 12-month storage at ‒20 °C is more than double that of other HRM reagents.

The passive reference dye may be added to fluorescence-based quantitative PCR assays to compensate for non-PCR related variations in fluorescence.