Spécifications

• Conjugaison:
  Unconjugated
• Type de protéine/peptide:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag
• Conditions de stockage:
  –20 °C
• Teneur en endotoxines:
  Less than 1,0 EU per μg by the LAL method
• Activité biologique:
  Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 5 μg/ml (100 μl/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0,3 - 2 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 10 μg/ml (100 μl/well) on Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-5 μg/ml (Routinely tested). Loaded Mouse Human chimeric MAb (5G12, Human IgG1) on AHC Biosensor, can bind Human Siglec-15, His Tag (Cat. No. SG5-H52H3) with an affinity constant of 0,52 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• ID de gène:
  Q6ZMC9-1
• Synonyme de protéine:
  Siglec-15|SIGLEC-15|Siglec15|CD33L3|CD33 antigen-like 3|sialic acid-binding Ig-like lectin 15
• Nom de la protéine/du peptide:
  Siglec-15
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular weight:
  28,4 kDa
• Niveau d'endotoxine:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH 5,5. Normally trehalose is added as protectant before lyophilisation.
• Température de transport:
  RT