Kit illustra™ blood genomicPrep Mini Spin

Pour l'isolation rapide et reproductible d'un ADN génomique de grande qualité à partir de sang total, de couche leuco-plaquettaire, de moelle osseuse et d'érythrocytes nucléés.

• ADN génomique de haute qualité avec une proportion plus élevée d'ADN de poids moléculaire plus élevé et moins de cisaillement
• Application démontrée à divers types de sang total, y compris l'homme, le cheval, le lapin, le rat et la souris
• Facilité d'utilisation accrue avec des changements de volume de pipetage réduits, un réglage de centrifugation et des composants de kit codés par couleur
• Vérifié dans plusieurs applications en aval, y compris la PCR en temps réel, la PCR en point final, la PCR multiplex et les digestions par enzymes de restriction

Genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.

For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.

The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μL sample. Input sample volumes can vary from 50 μL to 1 mL.

The method uses a simple genomic DNA purification protocol. This protcol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. This results in high molecular weight DNA that can be used downstream (e.g., in bone marrow genomic DNA for next generation sequencing).

The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.

Ordering information: Kit contains lysis buffer type 15 (75 ml), wash buffer type 6 (12 ml), elution buffer type 5 (12 ml), elution buffer type 9 (7.5 ml), protein precipitation buffer type 1 (2×17.5 m)l, one vial DNase I, DNase reaction buffer type 1 (1.4 ml), illustra Mini columns (50 x DNA), illustra Mini columns (50 x RNA), collection tubes (200) and an instruction booklet.

Delivery information: Kit contains illustra blood genomicPrep spin columns prepacked with a novel silica membrane, lyophilised proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

Caution: Yields and purities of gDNA, total RNA, and total denatured proteins can vary with user and depend on the nature and condition of the input sample.