Alamar blue cell viability assay

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

• High sensitivity and linerarity
• Quantitative
• Non-toxic to cells
• Scalable
• No cell lysis
• Stable
• Flexible and versatile

The alamarBlue (resazurin) dye in its oxidised form is blue in colour and non-fluorescent. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent. The continued growth of viable cells maintain a reducing environment (fluorescent, red) and inhibition of growth maintains an oxidised environment (non-fluorescent, blue), which can be detected using a fluorescence or absorbance detector.

Monitor cell proliferation and cell viability for in vitro cytotoxicity studies (e.g., effects of environmental toxins, cytokines, drugs, siRNA/microRNA). Monitor cell viability in real time for 3D tissue engineering applications.