Lambda DNA/HindIII Marker

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

The marker can be labelled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labelled radioactively or non radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.

The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65 °C for 5 minutes and then cooling on ice for 3 minutes.