Specifications

Cdt:
1 KIT
Hôte:
Désignation:
BIOARRAY™ Low input RNA amplification and biotin labeling system
Seq:
02
Environmentally Preferable:

Specifications

About this item

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Single tube reaction for amplification and labeling
Generates sufficient aRNA for standard microarray analysis from as little as 20 ng of total input RNA
Superior 3’/5’ transcript ratios demonstrating efficient in vitro transcription
3’/5’ ratios significantly better, as the kit completes transcript through the 5’ end

This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 20 ng and up to 500 ng. Entire amplification reaction can be performed in a single tube and the biotin-labeled aRNA can be purified using either magnetic beads or purification columns. The system has been optimised for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and in vitro transcript labeling.

Delivery: The kit contains first strand buffer (FSB) (40 µl), first strand enzyme mix (FSE) (10 µl), second strand buffer mix (SSB) (30 µl), second strand enzyme mix (SSE) (20 µl), transcription buffer mix (TB) (250 µl), DTT (75 µl), enhancer cocktail (EC) (75 µl), T7 RNA polymerase (T7) (50 µl) and nuclease-free water (W) (1 ml).