Fixable viability staining kits
À propos de cet article
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.
- Highly dead-cell specific
- Covalent labelling with reactive dyes
- Withstand fixation and permeabilisation
- The most available colours
- Dye options designed for spectral flow
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
The dyes are named for their excitation/emission wavelengths. Choose from a wide variety of colours with fluorescence from UV to near-IR. Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 can be used for either flow cytometry or fluorescence microscopy. Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora. Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.
Kit Components:
Live-or-Dye™ Fixable Viability Dye
Anhydrous DMSO
: Number of assays is based on 1 ml reaction size (106cells); actual number of assays may vary depending on staining volume.
17 Options disponibles ci-dessous
Product Details & Documents
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.
- Highly dead-cell specific
- Covalent labelling with reactive dyes
- Withstand fixation and permeabilisation
- The most available colours
- Dye options designed for spectral flow
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labelling is extremely stable, allowing the cells to be fixed and permeabilised without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimised to maximise live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
The dyes are named for their excitation/emission wavelengths. Choose from a wide variety of colours with fluorescence from UV to near-IR. Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 can be used for either flow cytometry or fluorescence microscopy. Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora. Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.
Kit Components:
Live-or-Dye™ Fixable Viability Dye
Anhydrous DMSO
: Number of assays is based on 1 ml reaction size (106cells); actual number of assays may vary depending on staining volume.
Spécifications de la famille de produits
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Informations sur le produit
- DésignationLaser lineCdtDisponibilitéPrix
- Live-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nm355 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32002-T - Live-or-Dye™ 350/448, DAPI or violet emission filter, for flow cytometry, (λEx/λEm) 347/448 nm355 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
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Specifications
Cat. No.BTIU32002 - Live-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nm405 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32003-T - Live-or-Dye™ 405/452, pacific blue emission filter, for flow cytometry, (λEx/λEm) 408/452 nm405 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
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Specifications
Cat. No.BTIU32003 - Live-or-Dye™ 488/515, FITC emission filter, for flow cytometry and microscopy, (λEx/λEm) 490/515 nm488 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32004-T - Live-or-Dye™ 488/515, FITC emission filter, for flow cytometry, (λEx/λEm) 490/515 nm488 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
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Specifications
Cat. No.BTIU32004 - Live-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nm488 or 561 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32005-T - Live-or-Dye™ 568/583, PE emission filter, for flow cytometry and microscopy, (λEx/λEm) 562/583 nm488 or 561 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
Plus
Specifications
Cat. No.BTIU32005 - Live-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nm488 or 561 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32006-T - Live-or-Dye™ 594/614, PE-Texas red emission filter, for flow cytometry and microscopy, (λEx/λEm) 561/624 nm488 or 561 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
Plus
Specifications
Cat. No.BTIU32006 - Live-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nm633 or 640 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
Plus
Specifications
Cat. No.BTIU32007-T - Live-or-Dye™ 640/662, APC emission filter, for flow cytometry and microscopy, (λEx/λEm) 642/662 nm633 or 640 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
Plus
Specifications
Cat. No.BTIU32007 - Live-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nm633 or 640 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32008-T - Live-or-Dye™ 750/777, APC-Cy7 emission filter, for flow cytometry, (λEx/λEm) 755/777 nm633 or 640 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
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Specifications
Cat. No.BTIU32008 - Live-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nm405 nm50 TestsConnexion Pour la disponibilité1 * 50 Tests116,00 €Spécifications :
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Specifications
Cat. No.BTIU32009-T - Live-or-Dye™ 405/545, AmCyan emission filter, for flow cytometry, (λEx/λEm) 395/545 nm405 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
Plus
Specifications
Cat. No.BTIU32009 - Live-or-Dye™ 510/550, for spectral cytometry488 or 532 nm200 TestsConnexion Pour la disponibilité1 * 200 Tests385,00 €Spécifications :
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Specifications
Cat. No.32012.
#: 20864.320
Fournisseur: VWR Chemicals
#: 83640.320
Fournisseur: VWR Chemicals
#: 1.00030.2500
Fournisseur: Merck
#: 31770.294
Fournisseur: VWR Chemicals
#: 83645.320
Fournisseur: VWR Chemicals
#: 1.09439.1000
Fournisseur: Merck