SulfoLink™ Immobilisation Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

• Specific to sulphhydryl (-SH) groups
• Prepare and couple protein samples in three and half hours
• Flexible coupling conditions, use pH 7,5 to 9,0 aqueous buffers, organic solvent or denaturant
• Streamlined protocols for sample preparation, immobilisation, and affinity purification
• Immobilise 2 to 20 mg of protein per 2 ml column of SulfoLink™ coupling resin

When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink™ resin react specifically and efficiently with the exposed sulphhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.