Amine reactive biotinylation kits, EZ-Link™
À propos de cet article
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
NHS-esters of biotin are water-insoluble and membrane-permeable because they do not possess a charged group. They may be used for biotinylating internal as well as external cellular components. Since NHS-esters of biotin are water-insoluble, for reactions in aqueous solution they must first be dissolved in an organic solvent, such as DMSO and DMF.
Sulfo-NHS-esters are water soluble and useful for applications that cannot tolerate organic solvents. Sulfo-NHS-esters of biotin are recommended for use as cell surface biotinylation reagents. Because of the charged sulfonate group, these compounds do not penetrate the plasma membrane, thus sulfo-NHS-esters biotinylation is restricted to the cell surface.
EZ-Link™ Sulfo-NHS Biotinylation Kits: Complete kit with an optimised protocol for labelling a protein and determining how much biotin has been attached. Fast and efficient labelling procedure.
EZ-Link™ Sulfo-NHS-LC Biotinylation Kits: Complete kit with an optimised protocol for labelling a protein and determining how much biotin has been attached. Fast and efficient labelling procedure.
EZ-Link™ NHS-PEG4 Biotinylation Kits: PEG4-Biotin transfers water-solubility to biotinylated molecules.
EZ-Link™ Sulfo-NHS-SS Biotinylation Kits: Cleavable biotinylation reagent allows for later removal of the biotin label.
8 Options disponibles ci-dessous
Product Details & Documents
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
NHS-esters of biotin are water-insoluble and membrane-permeable because they do not possess a charged group. They may be used for biotinylating internal as well as external cellular components. Since NHS-esters of biotin are water-insoluble, for reactions in aqueous solution they must first be dissolved in an organic solvent, such as DMSO and DMF.
Sulfo-NHS-esters are water soluble and useful for applications that cannot tolerate organic solvents. Sulfo-NHS-esters of biotin are recommended for use as cell surface biotinylation reagents. Because of the charged sulfonate group, these compounds do not penetrate the plasma membrane, thus sulfo-NHS-esters biotinylation is restricted to the cell surface.
EZ-Link™ Sulfo-NHS Biotinylation Kits: Complete kit with an optimised protocol for labelling a protein and determining how much biotin has been attached. Fast and efficient labelling procedure.
EZ-Link™ Sulfo-NHS-LC Biotinylation Kits: Complete kit with an optimised protocol for labelling a protein and determining how much biotin has been attached. Fast and efficient labelling procedure.
EZ-Link™ NHS-PEG4 Biotinylation Kits: PEG4-Biotin transfers water-solubility to biotinylated molecules.
EZ-Link™ Sulfo-NHS-SS Biotinylation Kits: Cleavable biotinylation reagent allows for later removal of the biotin label.
Product Family Options
EZ-Link™ Sulfo-NHS Biotinylation Kits
Informations sur le produit
- DésignationCdtDisponibilitéPrix
- EZ-Link™ micro Sulfo-NHS biotinylation kit, includes no-Weigh™ Sulfo-NHS biotin (8×1 mg), Zeba™ desalting columns (2 ml), and BupH™ phosphate buffered saline (500 ml)1 KITConnexion Pour la disponibilité1 * 1 KIT498,00 €
- EZ-Link™ Sulfo-NHS biotinylation kit, includes Sulfo-NHS biotin (25 mg), reaction buffer (500 ml), Zeba™ spin columns (10), HABA dye, 10 mM (1 ml), and avidin, affinity purified (10 mg)1 KITConnexion Pour la disponibilité1 * 1 KIT500,00 €
EZ-Link™ Sulfo-NHS-LC Biotinylation Kits
Informations sur le produit
- DésignationCdtDisponibilitéPrix
- EZ-Link™ Sulfo-NHS-LC biotinylation kit, includes Sulfo-NHS biotin (25 mg), reaction buffer (500 ml), Zeba™ spin columns (10), HABA dye, 10 mM (1 ml), and avidin, affinity purified (10 mg)1 KITConnexion Pour la disponibilité1 * 1 KIT550,00 €
- EZ-Link™ Micro Sulfo-NHS-LC biotinylation kit, includes No-Weigh™ Sulfo-NHS-LC biotin (8×1 mg), Zeba™ desalting columns (2 ml), and BupH™ phosphate buffered saline (500 ml)1 KITConnexion Pour la disponibilité1 * 1 KIT546,00 €
EZ-Link™ NHS-PEG₄ Biotinylation Kits
Informations sur le produit
- DésignationCdtDisponibilitéPrix
- EZ-Link™ NHS-PEG4 biotinylation kit, includes NHS-PEG4 biotin (8×2 mg), BupH™ phosphate buffered saline (500 ml), Zeba™ spin columns (10), HABA dye, 10 mM (1 ml), and avidin, affinity purified (10 mg)1 KITConnexion Pour la disponibilité1 * 1 KIT421,00 €
- EZ-Link™ Micro NHS-PEG4 biotinylation kit, includes No-Weigh™ NHS-PEG4 biotin (8×1 mg), Zeba™ desalting columns (2 ml), and BupH™ phosphate buffered saline (500 ml)1 KITConnexion Pour la disponibilité1 * 1 KIT392,00 €
EZ-Link™ Sulfo-NHS-SS Biotinylation Kits
Informations sur le produit
- DésignationCdtDisponibilitéPrix
- EZ-Link™ Sulfo-NHS-SS biotinylation kit, includes Sulfo-NHS-SS biotin (25 mg), Zeba™ spin columns (10), HABA dye, 10 mM (1 ml), and avidin, affinity purified (10 mg)1 KITConnexion Pour la disponibilité1 * 1 KIT552,00 €
- EZ-Link™ Mico Sulfo-NHS-SS biotinylation kit, includes Sulfo-NHS-SS biotin (8×1 mg), Zeba™ desalting columns (2 ml), and BupH™ phosphate buffered saline (500 ml)1 KITConnexion Pour la disponibilité1 * 1 KIT526,00 €
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