Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media
17-0810-01
: Cytiva
:
- Granulométrie:90 µm
- Cdt:200 mL
- Désignation:Phenyl Sepharose™ CL-4B
- Plage de pH:3 - 12
- Matrix:Cross-linked agarose, 4%, spherical
- Ligand:Phenyl
- Température de stockage:4 - 30 °C
- Capacité de fixation:HSA (Mr 68 000) 15-20 mg/ml drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained resin
- Ligand density:40 μmole/ml drained medium
- Débit:150 cm/h
- Type:Liquid
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
- Phenyl immobilized on 4% cross-linked agarose
- Aromatic ligand
- Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
- Negligible volume variation due to changes in pH or ionic strength
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.