You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Western blotting detection reagents, ECL western blotting substrate
Supplier: Thermo Fisher Scientific
ECL™ Western blotting substrate is an entry-level Western blotting substrate.
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Western blot assay kits, ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.
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Western blotting kit, ECL substrate, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
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Western blot stripping buffer, restore PLUS™
Supplier: Thermo Fisher Scientific
Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.
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Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: Merck
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
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Immobilon™ Block Noise Canceling Reagents
Supplier: Merck
Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.
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Fast western blotting kit, SuperSignal™ west Pico, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.
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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto
Supplier: Thermo Fisher Scientific
The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.
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Fixateur en solution dans gel polyacrylamide ( point isoélectrique ) Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixateur en solution dans gel polyacrylamide ( point isoélectrique )
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Peroxidase Indicator Reagent
Supplier: SIGMA ALDRICH MICROSCOPY
It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.
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Western blot stripping buffer, Western ReProbe™
Supplier: G-Biosciences
Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities
Supplier: Merck
Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.
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Chemicon® ReBlot Plus Kits
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Western blots, pre-made, mouse subcellular fraction specific blot
Supplier: G-Biosciences
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
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Chemiluminescent detection reagents, Luminata™ Western HRP substrates
Supplier: Merck
Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
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Chemiluminescent detection reagents, Immobilon® Western HRP substrates
Supplier: Merck
Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.
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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.
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Kits d'immunotransfert, MaxTag™
Supplier: Rockland Immunochemicals
Ces kits précis et sensibles conviennent parfaitement à la méthode « Western blot » ou « dot blot ». Ils offrent une grande flexibilité avec deux substrats uniques : substrat TMB bleu clair et substrat DAB stable brun. Les composants ont des codes de couleurs pour assurer des résultats sans erreur. Grâce à MaxTag™, vous pouvez exécuter jusqu'à 40 (10×10 cm) immunotransferts.
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Nitrotyrosine BSA for immunoblotting experiments
Supplier: Cayman Chemical
This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.
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CYTAG™ CGH labelling kits
Supplier: ENZO LIFE SCIENCES
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
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IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.
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Single tissue blots, multiple species
Supplier: G-Biosciences
Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.
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Fast western blot kit, SuperSignal™ West Dura, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.
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Immunodetection reagents, femtoLUCENT™ PLUS
Supplier: G-Biosciences
The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.
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Cell extract HL60 (non-induced)
Supplier: ENZO LIFE SCIENCES
Whole-cell extract of human HL-60 leukemia cells.
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picoLUCENT™ PLUS-HRP Kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.