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Choose from a range of reagents designed to quickly and efficiently aid in delivering genes into mammalian cells, transforming them to perform certain desirable functions for use in the laboratory. Effectively transfect DNA and siRNA into mammalian cells good levels of cell viability thanks to low toxicity in all reagents. Easily alter cells for immediate use or to freeze for later procedures, and use specialized reagents for the production of particular proteins.
Transformation kit, EZ-Yeast™, MP Biomedicals
Supplier: MP Biomedicals
The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.
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Sets de solution d'électroporation, BTXpress™
Supplier: BTX
Le BTXpress™ est une solution tampon unique, développée pour transférer rapidement et efficacement des gènes dans les cellules de mammifères autrefois considérées comme 'dures à transfecter' chimiquement ou par le biais d'autres méthodes non virales. La transfection à l'aide de cette solution d'électroporation haute performance distribue aussi bien l'ADN que l'ARNsi dans les cellules de mammifères.
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Yeast transformation kit, Alkali-Cation™, MP Biomedicals
Supplier: MP Biomedicals
Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
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Electrofusion buffer, BTXpress Cytofusion® Medium C
Supplier: BTX
BTXpress Cytofusion® Medium C is an advanced electrofusion buffer designed especially for hybridoma production. The low conductivity buffer is specially formulated to minimise cell turbulence during cell alignment and heating during electrofusion, for robust cell fusion efficiency and high cell viability. BTXpress Cytofusion® Medium C is sterile filtered from the highest quality non animal, medical grade reagents.
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Transformation reagent, TB solution-II
Supplier: Thermo Fisher Scientific
For transformation of competent cells.
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Electroporation buffer, BTXpress Cytoporation® Medium T and T4
Supplier: BTX
BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.
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Yeast transformation kit, Fast Yeast Transformation™
Supplier: G-Biosciences
Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.
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Flatpack chambers
Supplier: BTX
Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.