The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

Imidazole-buffered saline (5X)

Supplier: Thermo Fisher Scientific

Washing buffer, Imidazole-buffered saline (5X)

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée

Supplier: MP Biomedicals

Tampon de migration, Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Separating or resolving buffer (4X)

Supplier: Thermo Fisher Scientific

Separating Buffer, Separating or Resolving Buffer, 4X

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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SSC Solution tampon saline 20x concentrée (sodium citrate)

Supplier: LONZA

Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)

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IEF Cathode buffer (10X) with lysine

Supplier: Thermo Fisher Scientific

Tampon de migration, IEF Cathode buffer (10X) with lysine

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IEF Sample buffer (4X) pH 3 to 7 with lysine

Supplier: Thermo Fisher Scientific

Loading Buffer, IEF Sample Buffer (4X) with Lysine

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Tampons IPG

Supplier: Cytiva

Les tampons IPG sont des concentrés tamponnés contenant un ampholyte, spécialement formulés pour une utilisation avec les gels Immobiline™ DryStrip.

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Tampon de migration, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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DALT Buffer kit for precast gels

Supplier: Cytiva

DALT Buffer kit is used together with DALT Gel 12,5, which comprises concentrated buffers for running the gel, gel buffer for inserting the gel into DALT precast gel cassette and sealing solution for application of the IPG strip. The buffer in the gel is part of a buffer system that gives longer shelf-life.

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PBS Solution tampon saline 10x concentrée (tamponnée phosphate)

Supplier: MP Biomedicals

Washing buffer, PBS Solution tampon saline 10x concentrée (tamponnée phosphate)

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Low pH SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

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DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.