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303 results for Protéines purification

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Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Ion exchange chromatography media, SOURCE™ 30S

Ion exchange chromatography media, SOURCE™ 30S

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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Gel filtration media, Sephadex™ G-75

Gel filtration media, Sephadex™ G-75

Supplier: Cytiva

Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

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Ion exchange chromatography media, SOURCE™ 15S

Ion exchange chromatography media, SOURCE™ 15S

Supplier: Cytiva

SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

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Affinity coupling media, EAH Sepharose™ 4B

Affinity coupling media, EAH Sepharose™ 4B

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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Kits d'étalonnage pour filtration sur gel

Kits d'étalonnage pour filtration sur gel

Supplier: Cytiva

Les kits d'étalonnage pour filtration sur gel conviennent à l'étalonnage simple et fiable des milieux de filtration sur gel haute résolution et aux colonnes telles que Superdex, Superose et Sephacryl.

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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Milieu de chromatographie d'affinité pour ions métalliques immobilisés (IMAC) Ni Sepharose™ excel

Milieu de chromatographie d'affinité pour ions métalliques immobilisés (IMAC) Ni Sepharose™ excel

Supplier: Cytiva

Ni Sepharose™ excel est un milieu IMAC pré-chargé avec des ions nickel très fortement liés à un ligand chélatant. Les échantillons qui provoquent habituellement une réextraction des ions métalliques peuvent donc être chargés sur le milieu. Ni Sepharose™ excel est principalement conçu pour la capture et la purification des protéines marquées à l'histidine sécrétées dans les surnageants de cultures cellulaires des cellules eucaryotes telles que les cellules d'insectes ou les cellules CHO.

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Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

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Affinity chromatography media, MabSelect Xtra™

Affinity chromatography media, MabSelect Xtra™

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Gel filtration media, Sephadex™ G-10

Gel filtration media, Sephadex™ G-10

Supplier: Cytiva

Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Ion exchange chromatography media, SP Sepharose™ High Performance

Ion exchange chromatography media, SP Sepharose™ High Performance

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

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Sepharose™ CL-4B Gel Filtration Base Matrix

Sepharose™ CL-4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Affinity chromatography media, Chelating Sepharose™ Fast Flow

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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SDS-PAGE Clean Up Kit

SDS-PAGE Clean Up Kit

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Affinity chromatography media, Lentil Lectin Sepharose™ 4B

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

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Affinity chromatography media, Capto™ L

Affinity chromatography media, Capto™ L

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

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Ion exchange chromatography media, Capto™ S ImpAct

Ion exchange chromatography media, Capto™ S ImpAct

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

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Ion exchange chromatography media, SOURCE™ 15Q

Ion exchange chromatography media, SOURCE™ 15Q

Supplier: Cytiva

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

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Ion exchange chromatography media, Q Sepharose™ High Performance

Ion exchange chromatography media, Q Sepharose™ High Performance

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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Gel de filtration, Sephadex™ G-50

Gel de filtration, Sephadex™ G-50

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

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Kit 2-D Clean-Up

Kit 2-D Clean-Up

Supplier: Cytiva

Le kit 2-D Clean-Up est conçu pour préparer les échantillons pour une électrophorèse 2D qui autrement produirait des cartes de spots 2D de faible qualité en raison d'une conductivité élevée, d'un taux élevé de substances interférantes ou d'une faible concentration en protéines. Les réactifs précipitent quantitativement les protéines et laissent les substances interférantes, telles que les détergents, les sels, les lipides, les acides phénoliques et nucléiques, dans la solution.

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