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Vous avez recherché : Protéines purification

Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Classic magnetic IP/Co-IP kit, Pierce™

Classic magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

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HA-Tag Magnetic IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

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Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Supplier: Thermo Fisher Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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BugBuster® Ni-NTA His•Bind® purification kit

Supplier: Merck Millipore (Novagen)

BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

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Spin purification kit for GST proteins, Pierce™

Spin purification kit for GST proteins, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ GST Spin Purification Kit provides a fast, easy to use format for purifying GST-fusion proteins from cellular lysates.

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Agarose coupling kits, HOOK™

Agarose coupling kits, HOOK™

Supplier: G-Biosciences

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

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Control agarose resin, Pierce™

Control agarose resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.

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Ni-NTA His•Bind® Resin

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

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S-protein agarose, Novagen

Supplier: Merck Millipore (Novagen)

S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

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D-Desthiobiotin

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

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Immobilised β-cyclodextrin

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

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Ni-NTA His-Bind® Superflow™ Resin

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

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Recombinant streptavidin

Recombinant streptavidin

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

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Novagen® GST Bind™ Resin, MilliporeSigma

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

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Recombinant protein G, peroxidase conjugated, Calbiochem®

Supplier: Merck Millipore (Oncogene)

Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.

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TCEP, neutral, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

A ready-to-use stable, neutral form of the disulfide-reducing agent TCEP, hydrochloride.

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Pierce™ Agarose ChIP Kit

Pierce™ Agarose ChIP Kit

Supplier: Thermo Fisher Scientific

Provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

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Sulphhydryl immobilisation kit for proteins and peptides

Sulphhydryl immobilisation kit for proteins and peptides

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

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HCCA matrix

Supplier: BRUKER DALTRONIK

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

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BTSNM support materials, Chelex beads, 0,4 g with buffer

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

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Streptavidin agarose

Supplier: Merck Millipore (Novagen)

Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

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Immobilised Catalase

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

 

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Coupling catalyst, GlycoLink™

Coupling catalyst, GlycoLink™

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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Co-immunoprecipitation (Co-IP) Kit, Pierce™

Co-immunoprecipitation (Co-IP) Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

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Mass spectrometry reagents, MALDI matrices, single-use

Mass spectrometry reagents, MALDI matrices, single-use

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

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Pegylation reagent, MT(PEG)₄ methyl-PEG-thiol compound

Pegylation reagent, MT(PEG)₄ methyl-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Methyl-PEG4-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Pierce™ F(ab')2 Preparation Kit

Supplier: Thermo Fisher Scientific

Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

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