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Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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HisPur™ cobalt superflow agarose, Pierce™

HisPur™ cobalt superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

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Monomeric avidin resin, UltraLink™, Pierce™

Monomeric avidin resin, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

UltraLink™ Monomeric Avidin resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobilisation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.

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Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Capacity Endotoxin Removal Resins are ideal for the removal of endotoxins from protein or antibody samples prior to cell transfection or animal injection.

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Monomeric avidin agarose kit, Pierce™

Monomeric avidin agarose kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

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Immobilisation kit, AminoLink™ Plus, Pierce™

Immobilisation kit, AminoLink™ Plus, Pierce™

Supplier: Thermo Fisher Scientific

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

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Glutathione magnetic agarose, Pierce™

Glutathione magnetic agarose, Pierce™

Supplier: Thermo Fisher Scientific

These beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.

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SulfoLink™ Coupling Resin

SulfoLink™ Coupling Resin

Supplier: Thermo Fisher Scientific

SulfoLink™ coupling resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilisation of cysteine-peptides and other sulfhydryl molecules.

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Affinity chromatography resin, CDI-activated agarose, Pierce™

Affinity chromatography resin, CDI-activated agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

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Pierce™ HisPur™ Cobalt Agarose Spin Kits for Antibody Purification

Pierce™ HisPur™ Cobalt Agarose Spin Kits for Antibody Purification

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Purification Kit is designed for the efficient purification of recombinant polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

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Iminobiotin agarose, Pierce™

Iminobiotin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

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Immunoprecipitation, magnetic ChIP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

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ReadiPrep™ Nickel NTA Magnetic Beads

Supplier: AAT BIOQUEST

ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.

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Immobilisation kit, AminoLink™, Pierce™

Immobilisation kit, AminoLink™, Pierce™

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

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Immobilised Streptavidin resin, UltraLink™, Pierce™

Immobilised Streptavidin resin, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilised onto a beaded acrylamide support. UltraLink™ beads are hydrophilic, charge free, highly crosslinked, rigid and durable which makes the affinity resin suitable for a variety of batch or column type affinity procedures that require medium pressure chromatography.

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HisPur™ Ni-NTA superflow agarose, Pierce™

HisPur™ Ni-NTA superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

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Maleimide-activated protein, NeutrAvidin™ Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

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Pierce™, NAb™ Affinity Purification Kits, Spin Kits for Antibody Purification

Pierce™, NAb™ Affinity Purification Kits, Spin Kits for Antibody Purification

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

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Affinity purification resins, UltraLink™ iodoacetyl resin

Affinity purification resins, UltraLink™ iodoacetyl resin

Supplier: Thermo Fisher Scientific

UltraLink™ iodoacetyl resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblisation of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.

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Pierce™ Conjugate Purification Kit

Pierce™ Conjugate Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

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Pierce™ SDS-PAGE Sample Prep Kit

Pierce™ SDS-PAGE Sample Prep Kit

Supplier: Thermo Fisher Scientific

Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

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High Select™ top14 abundant protein depletion resin, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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Classic IP kit, Pierce™

Classic IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

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Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Supplier: Thermo Fisher Scientific

Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

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F(ab')2 Micro preparation kit, Pierce™

F(ab')2 Micro preparation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

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IgM purification kit , Pierce™

IgM purification kit , Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ IgM Purification Kit is an easy to use affinity matrix and buffer system to purify IgM from mouse ascites fluid. The method uses immobilised mannan binding protein (MBP), which binds to IgM in the kit-supplied buffers.

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Immobilisation kit for peptides, SulfoLink™, Pierce™

Immobilisation kit for peptides, SulfoLink™, Pierce™

Supplier: Thermo Fisher Scientific

Immobilisation Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

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Fab Preparation kits, Pierce™

Fab Preparation kits, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Fab preparation kits use immobilised papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

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Pierce™ Protein A/G Coated Plate IP Kit

Pierce™ Protein A/G Coated Plate IP Kit

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G coated plate IP kit uses coated protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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