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Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Protease affinity chromatography media, PreScission™, Cytiva

Protease affinity chromatography media, PreScission™, Cytiva

Supplier: Cytiva

Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.

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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

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Gel filtration media, Sephadex™ G-15

Gel filtration media, Sephadex™ G-15

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Ion exchange chromatography media, SP Sepharose™ High Performance

Ion exchange chromatography media, SP Sepharose™ High Performance

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

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Gel de filtration, Sephadex™ G-50

Gel de filtration, Sephadex™ G-50

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

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Multimodal chromatography media, Capto™ Core 700

Multimodal chromatography media, Capto™ Core 700

Supplier: Cytiva

Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).

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Gelatin-Sepharose® 4B

Gelatin-Sepharose® 4B

Supplier: Cytiva

Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Multimodal chromatography media, Capto™ MMC

Multimodal chromatography media, Capto™ MMC

Supplier: Cytiva

Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

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Sepharose™ CL-4B Gel Filtration Base Matrix

Sepharose™ CL-4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Gel filtration media, Sephadex™ G-10

Gel filtration media, Sephadex™ G-10

Supplier: Cytiva

Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Affinity chromatography media, Chelating Sepharose™ Fast Flow

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Affinity chromatography media, MabSelect Xtra™

Affinity chromatography media, MabSelect Xtra™

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Kits d'étalonnage pour filtration sur gel

Kits d'étalonnage pour filtration sur gel

Supplier: Cytiva

Les kits d'étalonnage pour filtration sur gel conviennent à l'étalonnage simple et fiable des milieux de filtration sur gel haute résolution et aux colonnes telles que Superdex, Superose et Sephacryl.

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Milieux de filtration sur gel, Sephadex™ LH-20

Milieux de filtration sur gel, Sephadex™ LH-20

Supplier: Cytiva

Sephadex™ LH-20 est un milieu de chromatographie liquide destiné au classement de taille moléculaire des produits naturels tels que les stéroïdes, les terpénoïdes, les lipides, les peptides de faible poids moléculaire. Utile pour la préparation à l'échelle analytique et industrielle des espèces moléculaires étroitement apparentées.

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Accessoires et pièces détachées pour ÄKTApilot
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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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Ion exchange chromatography media, Capto™ S ImpAct

Ion exchange chromatography media, Capto™ S ImpAct

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

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SDS-PAGE Clean Up Kit

SDS-PAGE Clean Up Kit

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

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Ion exchange chromatography media, SOURCE™ 15Q

Ion exchange chromatography media, SOURCE™ 15Q

Supplier: Cytiva

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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SP Sepharose™ XL Ion Exchange Chromatography Media

SP Sepharose™ XL Ion Exchange Chromatography Media

Supplier: Cytiva

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

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Affinity chromatography media, MabSelect SuRe™ LX

Affinity chromatography media, MabSelect SuRe™ LX

Supplier: Cytiva

MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

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Reversed phase chromatography media, SOURCE™ 15RPC

Reversed phase chromatography media, SOURCE™ 15RPC

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

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Affinity coupling media, CNBr-activated Sepharose™ 4

Affinity coupling media, CNBr-activated Sepharose™ 4

Supplier: Cytiva

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

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Milieux de chromatographie par échange d'ions, CM Sepharose™ Fast Flow

Milieux de chromatographie par échange d'ions, CM Sepharose™ Fast Flow

Supplier: Cytiva

CM Sepharose™ Fast Flow est un échangeur de cations faible basé sur la plateforme d'échange d'ions bien établie Sepharose™ Fast Flow, largement utilisée pour les séparations de protéines préparatives, tant dans le domaine de la recherche que dans les applications industrielles.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.

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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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