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Vous avez recherché : Protéines purification

Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Sulphhydryl immobilisation kit for proteins and peptides

Sulphhydryl immobilisation kit for proteins and peptides

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

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Immobilised β-cyclodextrin

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

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Novagen® GST Bind™ Resin, MilliporeSigma

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

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Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

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Affinity chromatography media, Dextrin Sepharose™ High Performance

Affinity chromatography media, Dextrin Sepharose™ High Performance

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

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Affinity chromatography media, Glutathione Sepharose™ High Performance

Affinity chromatography media, Glutathione Sepharose™ High Performance

Supplier: Cytiva

Idéal pour une purification performante des protéines taguées GST.

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Affinity chromatography media, Capto™ Blue

Affinity chromatography media, Capto™ Blue

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

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Affinity chromatography media, IMAC Sepharose™ High Performance

Affinity chromatography media, IMAC Sepharose™ High Performance

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

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Sepharose Chromatography Resins, Strep-Tactin® XT

Sepharose Chromatography Resins, Strep-Tactin® XT

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

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Ion exchange chromatography media, Capto™ Q ImpRes

Ion exchange chromatography media, Capto™ Q ImpRes

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Milieux de chromatographie d'affinité, TALON® Superflow™

Milieux de chromatographie d'affinité, TALON® Superflow™

Supplier: Cytiva

Milieux de chromatographie à base de cobalt conçus pour la purification des protéines marquées à l'histidine par chromatographie d'affinité pour ions métalliques immobilisés (IMAC).

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Ion exchange chromatography media, Capto™ DEAE

Ion exchange chromatography media, Capto™ DEAE

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Hydrophobic interaction chromatography media, Capto™ Butyl

Hydrophobic interaction chromatography media, Capto™ Butyl

Supplier: Cytiva

Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.

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Multimodal chromatography media, Capto™ adhere

Multimodal chromatography media, Capto™ adhere

Supplier: Cytiva

Capto™ adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process.

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Affinity chromatography media, Glutathione Sepharose™ 4B

Affinity chromatography media, Glutathione Sepharose™ 4B

Supplier: Cytiva

Purification rapide et simple des protéines taguées provenant de lysats bactériens, de protéines glutathione dépendantes ou de Gluthatione S transférase.

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Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.

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Hydrophobic interaction chromatography media, Capto™ SP ImpRes

Hydrophobic interaction chromatography media, Capto™ SP ImpRes

Supplier: Cytiva

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Gel filtration media, Sephacryl™ High Resolution

Gel filtration media, Sephacryl™ High Resolution

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

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Ion exchange chromatography media, Capto™ S

Ion exchange chromatography media, Capto™ S

Supplier: Cytiva

Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Ion exchange chromatography media, Capto™ Q

Ion exchange chromatography media, Capto™ Q

Supplier: Cytiva

Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow

Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow

Supplier: Cytiva

Purification rapide et en une seule étape des protéines taguées GST ou autres protéines glutathione dépendantes ou la Glutathione S transférase.

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Coupling catalyst, GlycoLink™

Coupling catalyst, GlycoLink™

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Supplier: Cytiva

Pour la séparation des protéines selon une gamme de pH et des purifications à partir d'échantillons bruts. Disponible avec de forts échangeurs d'anions et de cations :

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Affinity chromatography media, Ni Sepharose™ High Performance

Affinity chromatography media, Ni Sepharose™ High Performance

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

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Milieux de chromatographie d'affinité, Ni Sepharose™ 6 Fast Flow

Milieux de chromatographie d'affinité, Ni Sepharose™ 6 Fast Flow

Supplier: Cytiva

Ni Sepharose™ 6 Fast Flow est un milieu de biopurification qui se compose de billes d'agarose réticulée à 6% modifiées par un ligand chélatant immobilisé sur la matrice de base et pré-chargé avec des ions Ni²⁺ pour la purification des protéines marquées à l'histidine.

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Mass spectrometry reagents, MALDI matrices, single-use

Mass spectrometry reagents, MALDI matrices, single-use

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

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Pegylation reagents, methyl-PEG-amine compounds

Pegylation reagents, methyl-PEG-amine compounds

Supplier: Thermo Fisher Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Recombinant streptavidin

Recombinant streptavidin

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

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Recombinant protein G, peroxidase conjugated, Calbiochem®

Supplier: Merck Millipore (Oncogene)

Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.

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Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

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