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Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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GlycoLink™ IP kit

GlycoLink™ IP kit

Supplier: Thermo Fisher Scientific

Thermo Scientific™ GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilisation of antibodies and other glycoproteins via oxidised sugar groups.

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High Select™ HSA/immunoglobulin depletion resin

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select HSA/Immunoglobulin Depletion Resin decreases the albumin and antibody components that are abundant in human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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Monomeric avidin agarose, Pierce™

Monomeric avidin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

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Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

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Coupling resin, CarboxyLink™

Coupling resin, CarboxyLink™

Supplier: Thermo Fisher Scientific

CarboxyLink™ coupling resin is used for covalent immobilisation of peptides or other carboxyl-containing molecules to a porous, beaded resin for use in affinity purification procedures.

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TOYOPEARL® Super A Affinity Chromatography Protein Resins

Supplier: TOSOH Bioscience

TOYOPEARL® Super A resin provides a cutting-edge experience in protein A media, blending top-tier critical performance attributes with exceptional operational flexibility. An efficient and durable protein A resin.

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Classic magnetic IP/Co-IP kit, Pierce™

Classic magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

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Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.

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Immobilisation kit for peptides, SulfoLink™, Pierce™

Immobilisation kit for peptides, SulfoLink™, Pierce™

Supplier: Thermo Fisher Scientific

Immobilisation Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

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Direct magnetic IP/Co-IP kit, Pierce™

Direct magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

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Pierce™ Avidin Agarose

Pierce™ Avidin Agarose

Supplier: Thermo Fisher Scientific

Pierce™ Avidin agarose is purified avidin immobilised on crosslinked 6% beaded agarose for the affinity purification of biotinylated macromolecules.

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Pierce™ Conjugate Purification Kit

Pierce™ Conjugate Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

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HA-Tag Magnetic IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

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Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Immunoprecipitation, magnetic IP/Co-IP kit, c-Myc-tag, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Supplier: Thermo Fisher Scientific

Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

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Affinity purification resins, UltraLink™ iodoacetyl resin

Affinity purification resins, UltraLink™ iodoacetyl resin

Supplier: Thermo Fisher Scientific

UltraLink™ iodoacetyl resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblisation of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.

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AminoLink™ Plus micro immobilisation kit, Pierce™

AminoLink™ Plus micro immobilisation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ AminoLink™ Plus Micro immobilisation kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

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Recombinant protein A, Pierce™

Recombinant protein A, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Recombinant protein A/G, Pierce™

Recombinant protein A/G, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Recombinant protein L, Pierce™

Recombinant protein L, Pierce™

Supplier: Thermo Fisher Scientific

Purified (unconjugated) Pierce™ Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.

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ReadiPrep™ Nickel NTA Magnetic Beads

Supplier: AAT BIOQUEST

ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.

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Iminobiotin agarose, Pierce™

Iminobiotin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

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Glutathione magnetic agarose, Pierce™

Glutathione magnetic agarose, Pierce™

Supplier: Thermo Fisher Scientific

These beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.

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BugBuster® Ni-NTA His•Bind® purification kit

Supplier: Merck Millipore (Novagen)

BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

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Ni-NTA His•Bind® Resin

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

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Systèmes de purification des protéines, ÄKTA™ start

Systèmes de purification des protéines, ÄKTA™ start

Supplier: Cytiva

ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.

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Maleimide-activated protein, NeutrAvidin™ Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

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Agarose coupling kits, HOOK™

Agarose coupling kits, HOOK™

Supplier: G-Biosciences

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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