Vous avez recherché : Protéines purification

Supplier: Thermo Fisher Scientific

Pierce™ Protein A Plus agarose is a high capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: AAT BIOQUEST

ReadiPrep™ Protein G-Agarose Resin is a high-quality affinity resin designed for the isolation and purification of immunoglobulin classes, subclasses, and fragments from mammalian serum and biological fluids.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G UltraLink™ and Protein A/G Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which recombinant Protein A/G has been immobilised for use in IgG purification methods. Immoblised proteins are covalently coupled to an agarose support to provide an efficient method of purifying antibodies.

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G plus agarose is a high capacity agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

SulfoLink™ Immobilisation Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Supplier: G-Biosciences

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.

Supplier: Thermo Fisher Scientific

The GlycoLink™ Immobilisation Kit contains affinity columns and reagents to immobilise glycoproteins through oxidised sugar groups for use in affinity-purification applications or studies of protein-protein interactions. This kit is ideal for purification of antigens using covalently coupled antibodies, determining binding partners for a glycoprotein of interest and for performing large-scale affinity purifications.

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates.

Supplier: Thermo Fisher Scientific

NeutrAvidin™ agarose is a beaded agarose resin of immobilised NeutrAvidin™ protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Thiophilic adsorbent is a protein-free immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human. This Pierce™ Thiophilic Adsorption Kit is a purification kit containing columns pre-packed with the resin and ready-to-use buffers.

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin agarose is a standard-capacity crosslinked 6% beaded agarose resin of immobilised recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilised for use in IgG purification applications where the native ligand has undesirable effects.

Supplier: Thermo Fisher Scientific

UltraLink™ Monomeric Avidin resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobilisation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

Supplier: Thermo Fisher Scientific

Pierce™ Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Supplier: Thermo Fisher Scientific

The Pierce™ IgM Purification Kit is an easy to use affinity matrix and buffer system to purify IgM from mouse ascites fluid. The method uses immobilised mannan binding protein (MBP), which binds to IgM in the kit-supplied buffers.

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

Supplier: Abcam

Sample Diluent NBS (ab221827) is part of the reagents, controls and accessories range. High-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates are available.

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Abcam

Sample Diluent NBP is part of the reagents, controls and accessories range.