Kits de purification des protéines

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

Supplier: Abcam

TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants.

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Supplier: AGILENT

The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).

Supplier: G-Biosciences

For the rapid purification of soluble, 6X His or GST tagged proteins.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

Supplier: G-Biosciences

Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Supplier: G-Biosciences

STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

Supplier: Merck

Le système de purification Amicon® Pro est un outil centrifuge permettant la purification des protéines par concentration et échange de tampon.

Supplier: Abcam

The Antibody Concentration And Clean-Up Kit method allows the user to : -easily and quickly remove excess buffer from the antibody for a more concentrated antibody solution. -perform a simple buffer exchange to transfer the antibody into a more favorable buffer for conjugation

Supplier: Abcam

Antibodies are sometimes only available at concentrations that are too low for antibody conjugation or other antibody applications.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Supplier: AGILENT

Starter reagent kit for multiple affinity removal LC columns.

Supplier: Abcam

Abcam's BSA Removal Kit( ab173231) offers: Efficiency: It provides a simple one-step, 10-minute method to effectively separate BSA from antibodies. Versatility: The kit can be used on any antibody subtype and species, making it highly adaptable for various research needs. Convenience: The antibody is left in a suitable position for transfer to a buffer more suited to conjugation, streamlining the preparation process.

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.