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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Compat-Able™ Protein assay preparation reagent set
Supplier: Thermo Fisher Scientific
The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
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Coomassie Plus™ protein assay kit, Compat-Able™
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
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Firefly luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
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Coomassie (Bradford) protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
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Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Tetracysteine REASH Reagent *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.
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Pierce™ Protease Assay Kits, Colorimetric and Fluorometric
Supplier: Thermo Fisher Scientific
Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.
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Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible
Supplier: Thermo Fisher Scientific
This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).
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Biotinylated-protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.
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Non-interfering Protein Assay™ kit, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.
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Quantitative colorimetric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
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Streptavidin poly-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.
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Glutathione Fluorometric Assay Kit
Supplier: Abcam
Simple assay to detect total glutathione.
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Protein Assay, Protn-Latex™
Supplier: G-Biosciences
A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
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Biotin labelling kit
Supplier: Abnova
Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.
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Kit 2-D Quant
Supplier: Cytiva
Kit développé pour la détermination de protéines concentrées à partir de gels d'électrophorèse 2-D, SDS-PAGE ou IEF.
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Histone, from calf thymus, MP Biomedicals
Supplier: MP Biomedicals
Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.
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Supplier: GRANT INSTRUMENTS
Supplier: BOCHEM
Supplier: VWR Collection
Supplier: OMEGA BIO-TEK
Supplier: DWK Life Sciences
Supplier: Thermo Fisher Scientific
Supplier: VWR Collection
Supplier: Oxford Nanopore Technologies
Supplier: Industrial Physics
Supplier: AAT BIOQUEST
Supplier: Integra
Supplier: Palbam
Supplier: VWR Collection
Supplier: Integra
Supplier: VWR Collection
Supplier: FACELLITATE
Supplier: NANOENTEK