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Tests enzymatiques

Les dosages enzymatiques, qui mesurent la consommation du substrat ou la production totale, permettent de suivre l'activité enzymatique. Avec les réactifs et le matériel nécessaires, quelle que soit la méthode choisie, les études de cinétique enzymatique et d'inhibition peuvent être réalisées avec succès. Les méthodes d'échantillonnage en continu fournissent des mesures d'activité tout au long du processus, tandis que les techniques discontinues effectuent les calculs après l'arrêt de la réaction. Les dosages enzymatiques standardisés garantissent l'identification et le dosage précis des molécules de protéines présentes dans les échantillons.

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PKC kinase activity kit

Supplier: Enzo Life Sciences

The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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ProteoStat™ PDI assay kit

Supplier: Enzo Life Sciences

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

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Complex I Enzyme Activity Dipstick Assay Kit

Complex I Enzyme Activity Dipstick Assay Kit

Supplier: Abcam

Complex I Enzyme Activity Dipstick Assay kit is an immunoassay kit for the detection of Complex I Enzyme Activity in Mouse, Rat, Cow, Human in cell culture extracts, tissue, cell culture supernatant samples.

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Phosphatase assay kit, BIOMOL® Green

Phosphatase assay kit, BIOMOL® Green

Supplier: Enzo Life Sciences

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

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Neutrophil Elastase colorimetric drug discovery kit

Neutrophil Elastase colorimetric drug discovery kit

Supplier: Enzo Life Sciences

Neutrophil Elastase Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.

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Matrix Metalloproteinase-12 (MMP-12) colorimetric drug discovery kit

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-12 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-12 (MMP-12, macrophage elastase, metalloelastase) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96-well plate format. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH), and a detailed instruction booklet.

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MMP Inhibitor profiling kit, fluorometric

Supplier: Enzo Life Sciences

A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a sensitive fluorogenic substrate . Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.

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Akt kinase activity kit

Supplier: Enzo Life Sciences

This is a non-radioactive Akt kinase activity assay, providing a safe, rapid and reliable method for the screening of inhibitors or activators of Akt and for quantitating the activity of Akt in purified or partially purified enzyme preparations. The Akt Kinase activity kit is based on a solid phase enzyme immunoassay (EIA) that utilizes a specific synthetic peptide as a substrate for Akt and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of Akt (1, 2, 3) activity in the solution phase.

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Catalase Assay Kit

Catalase Assay Kit

Supplier: Cayman Chemical

For the measurement of catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.

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Matrix Metalloproteinase-9 (MMP-9) colorimetric drug discovery kit

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-9 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a thiopeptide as a chromogenic substrate.

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Ca2+/Calmodulin Solution 10x, Abcam

Supplier: Abcam

10x stock solution used for assaying protein kinase activity of CAMK family members.

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Caspase-2 fluorometric assay kit

Supplier: Enzo Life Sciences

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

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Matrix Metalloproteinase-1 (MMP-1) fluorometric drug discovery kit

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-1 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Matrix Metalloproteinase-12 (MMP-12) fluorometric drug discovery kit

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-12 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-12 (MMP-12, metalloelastase, macrophage elastase) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH.

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Matrix Metalloproteinase-7 (MMP-7) colorimetric drug discovery kit

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.

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Cathepsin K drug discovery kit

Supplier: Enzo Life Sciences

The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.

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