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Tests cellulaires

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit, Optimized for Difficult Cell Lines

Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit, Optimized for Difficult Cell Lines

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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Nuclear Red™ DCS1

Nuclear Red™ DCS1

Supplier: AAT BIOQUEST

Our Nuclear Red™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analysing DNA content in dead, fixed or apoptotic cells.

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Nuclear Blue™ DCS1

Nuclear Blue™ DCS1

Supplier: AAT BIOQUEST

Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells.

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Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit

Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit

Supplier: AAT BIOQUEST

Calcium flux assays are the preferred methods for screening G protein coupled receptors (GPCRs) in drug discovery.

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Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labelling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

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CytoScan™ LDH Cytotoxicity Assay kit

CytoScan™ LDH Cytotoxicity Assay kit

Supplier: G-Biosciences

CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.

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Test à tube unique de luciférine de luciole et de rénilla

Test à tube unique de luciférine de luciole et de rénilla

Supplier: Biotium

Ce kit est conçu pour mesurer les activités de luciférine de luciole et de rénilla dans des tests de cellules transfectées. La luciférine de luciole est largement utilisée en tant que rapporteur pour étudier la régulation et la fonction des gènes, ainsi que pour le criblage pharmaceutique. C’est un rapporteur génétique très sensible en raison de l’absence d’activité luciférine endogène dans les cellules ou les tissus de mammifères. La luciférine de rénilla a été utilisée comme gène rapporteur pour étudier la régulation et la fonction des gènes in vitro et in vivo. Elle est couramment utilisée dans les tests multiplexeurs de transcription ou comme contrôle de normalisation de la transfection pour les tests de luciférine de luciole.

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Firefly luciferase assay kit 2.0

Supplier: Biotium

This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.

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BCA protein assay kit

Supplier: Merck Millipore (Novagen)

BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

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Amyloid plaque staining reagent, Amylo-Glo® RTD™

Amyloid plaque staining reagent, Amylo-Glo® RTD™

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

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Alamar blue cell viability assay

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

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Amyloid plaque stain reagent, with EtBr counter stain, Amylo-Glo® RTD™

Amyloid plaque stain reagent, with EtBr counter stain, Amylo-Glo® RTD™

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

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Yeast viability staining kits

Yeast viability staining kits

Supplier: Biotium

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

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CellBrite™ Fix membrane stains, Biotium

CellBrite™ Fix membrane stains, Biotium

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

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Fixable viability staining kits

Fixable viability staining kits

Supplier: Biotium

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

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Firefly luciferase assay kit

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

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Live-or-Dye™ Yeast fixable live/dead staining kit

Live-or-Dye™ Yeast fixable live/dead staining kit

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

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Annexin V-FITC apoptosis detection kit II, Sigma-Aldrich®

Supplier: Merck Millipore (Oncogene)

Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.

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Red/green (FUN®1), ViaVac™

Red/green (FUN®1), ViaVac™

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Yeast vitality staining kit

Yeast vitality staining kit

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Live-or-Dye NucFix™ red staining kit

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

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BCA protein assay kit

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

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Nrf2 transcription factor assay kit

Nrf2 transcription factor assay kit

Supplier: Cayman Chemical

Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.

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ATP assay kit

ATP assay kit

Supplier: Abnova

ATP Assay Kit is for the rapid bioluminescent determination of ATP in biological samples.

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Antioxidant assay kit

Antioxidant assay kit

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

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Malachite green phosphate assay kit

Malachite green phosphate assay kit

Supplier: Abnova

Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.

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Cell proliferation assay kit, WST-1

Cell proliferation assay kit, WST-1

Supplier: Abnova

WST-1 cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model.

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Myco-Sniff-Rapid™ Mycoplasma Luciferase Detection Kits, MP Biomedicals

Myco-Sniff-Rapid™ Mycoplasma Luciferase Detection Kits, MP Biomedicals

Supplier: MP Biomedicals

A rapid, simple and sensitive method for detecting mycoplasma contamination in cell cultures.

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Nitric oxide assay kit

Nitric oxide assay kit

Supplier: Abnova

Nitric Oxide Assay Kit for the quantitative colorimetric determination of nitric oxide in cell, food sample, plasma, serum, tissue and urine.

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Methylated DNA capture kit

Supplier: Abnova

Methylated DNA Capture Kit is used to enrich methylated DNA.

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