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Tests cellulaires

Tests cellulaires

Les essais cellulaires ont toujours été un outil puissant dans le laboratoire de recherche. Ils sont extrêmement polyvalents et peuvent être conçus pour mesurer pratiquement toute fonction cellulaire ou biochimique. La détection sensible de sondes fluorescentes aide à évaluer la signalisation cellulaire et les voies de mort cellulaire, ce qui est utile pour la quantification rapide de la viabilité cellulaire. Les essais cellulaires peuvent être utilisés pour mesurer les effets de différents traitements sur la viabilité cellulaire et pour évaluer les effets toxiques médiés par divers mécanismes.

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Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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Membrane potential detection kit, MITO-ID®

Membrane potential detection kit, MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

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LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

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CF® Dye Dextran 250000 MW, anionic and fixable

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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Apoptosis and necrosis quantitation kits

Apoptosis and necrosis quantitation kits

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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ATP-Glo™ Bioluminometric cell viability assay

ATP-Glo™ Bioluminometric cell viability assay

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

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CF® Dye Dextran 70000 MW, anionic and fixable

CF® Dye Dextran 70000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

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Kit de test Steady-Luc™ Firefly HTS (lyophilisé)

Kit de test Steady-Luc™ Firefly HTS (lyophilisé)

Supplier: Biotium

La luciférine de luciole est largement utilisée comme gène rapporteur de transcription pour la recherche et le criblage de médicaments. Le kit Steady-Luc™ HTS peut être utilisé avec des lecteurs de plaque à luminescence sans systèmes d’injection d’échantillons ou pour le criblage à haut débit.

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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Supplier: Enzo Life Sciences

A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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Phagocytosis assay kit (IgG FITC)

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

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ViaFluor® 488 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with green fluorescence.

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CF® Dye Dextran 10000 MW, anionic and fixable

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

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ER-ID™ red assay kit (GFP-CERTIFIED®)

ER-ID™ red assay kit (GFP-CERTIFIED®)

Supplier: Enzo Life Sciences

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

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Blue IHC chromogen (AP), HIGHDEF®

Supplier: Enzo Life Sciences

Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

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FLUOR DE LYS® HDAC fluorometric activity assay kit

Supplier: Enzo Life Sciences

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

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