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Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Buccutite™ Poly-HRP Antibody Conjugation Kit

Buccutite™ Poly-HRP Antibody Conjugation Kit

Supplier: AAT BIOQUEST

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

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ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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ReadiLink™ Rapid XFD750 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

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Helixyte™ iFluor® 555 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 555 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 555 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Supplier: Thermo Fisher Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

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Crosslink immunoprecipitation (IP) kit, Pierce™

Crosslink immunoprecipitation (IP) kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Biotin-NHS

Supplier: Merck Millipore (Oncogene)

Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

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Ghost Dye™ Red 710

Ghost Dye™ Red 710

Supplier: Tonbo Biosciences

Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.

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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Bilirubin conjugate, ditaurate, disodium salt, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Bilirubin conjugate, ditaurate is suitable as a direct bilirubin standard. It behaves similarly to purified bilirubin glucuronides from human bile in diazo tests.

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AZOCOLL™ substrate, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.

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Biotinylated heparin, porcine intestinal mucosa, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Biotinylated heparin contains 5 − 8 mole-percent biotin. Biotin attachment is through a 12-atom spacer arm containing a cleavable disulfide group.

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Antibody labelling kits, Mix-n-Stain™ DNP

Supplier: Biotium

Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.

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Fluor-free acids, DyLight™

Fluor-free acids, DyLight™

Supplier: Thermo Fisher Scientific

DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

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Antibody labelling kits, Mix-n-Stain™ Digoxigenin

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.

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Streptavidin, maleimide-activated

Streptavidin, maleimide-activated

Supplier: Thermo Fisher Scientific

Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.

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Biotin NTA

Biotin NTA

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

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Peptide coupling kits, HOOK™

Peptide coupling kits, HOOK™

Supplier: G-Biosciences

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.

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Biotin kits, HOOK™

Biotin kits, HOOK™

Supplier: G-Biosciences

HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.

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FluorSave™ reagent, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.

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Immunostainer enhancer, Pierce™

Immunostainer enhancer, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

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VeriFluor™ Far-Red Passive Reference Dye

Supplier: Biotium

A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.

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Reactive Dye 5-packs, Amersham CyDye™

Reactive Dye 5-packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.

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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

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Nucléotides fluorescents, Amersham™ CyDye™

Nucléotides fluorescents, Amersham™ CyDye™

Supplier: Cytiva

Pour étiquetage des sondes d'ADN et d'ARN, notamment pour les analyses multicolores sur des microéchantillons, FISH, identification chromosomique, peinture chromosomique, établissement de caryotype et cartographie génétique.

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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Supplier: Cytiva

Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.

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Colorants mono-réactifs Amersham Cy3 et Cy5, paquets de 5, Cytiva

Colorants mono-réactifs Amersham Cy3 et Cy5, paquets de 5, Cytiva

Supplier: Cytiva

Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Protein labelling Kit, Amersham™ QuickStain

Protein labelling Kit, Amersham™ QuickStain

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

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