Vous avez recherché : Systèmes de liaison et de marquage des protéines
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
Expand 1 Items
Kits de marquage des anticorps, colorant Mix-n-Stain™ CF™
Supplier: Biotium
Les kits de marquage des anticorps Mix-n-Stain™ CF™ permettent de simplifier la préparation des anticorps marqués par fluorescence.
Expand 87 Items
CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
Expand 4 Items
ZnAF-2.tetrahydrochloride
Supplier: ENZO LIFE SCIENCES
Fluorescent probe for Zn2+
Expand 1 Items
femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
Expand 5 Items
SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
Expand 2 Items
Avidin, biotin, protein A/G and streptavidin reagents
Supplier: Rockland Immunochemicals
Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.
Expand 24 Items
Mix-n-Stain™ Cyanine dye antibody labelling kits
Supplier: Biotium
Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.
Expand 7 Items
Biotin reagents, OneQuant™ HOOK™
Supplier: G-Biosciences
The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.
Expand 6 Items
CF™ azide reactive dyes
Supplier: Biotium
CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
Expand 3 Items
femtoCHROMO™- HRP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.
Expand 5 Items
CF® Dye dCTP conjugates
Supplier: Biotium
CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
Expand 18 Items
Nick Translation DNA Labelling System 2.0
Supplier: ENZO LIFE SCIENCES
The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.
Expand 1 Items
Organelle-ID™ RGB reagent IV
Supplier: ENZO LIFE SCIENCES
Organelle-ID RGB® reagent IV is a mixture of cell-permeable red fluorescent endoplasmic reticulum dye, green fluorescent lysosomal dye, and blue fluorescent nucleic acid dye.
Expand 1 Items
HRP - conjugate stabiliser, SeramunStab® STC
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.
Expand 1 Items
Bio-16-dUTP
Supplier: ENZO LIFE SCIENCES
Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
Expand 1 Items
AMC (7-Amino-4-methylcoumarin) standard
Supplier: ENZO LIFE SCIENCES
Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.
Expand 1 Items
Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)
Supplier: ENZO LIFE SCIENCES
Cell viability dye
Expand 1 Items
Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
Expand 15 Items
Coelenterazine Substrates for Renilla Luciferase and Apoaequorin
Supplier: Biotium
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.
Expand 39 Items
Gold 525 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Gold 525 dUTP (lyophilised)
Expand 1 Items
R-Phycoerythrin (RPE)
Supplier: Columbia Biosciences
Many applications and instruments were developed specifically for R-phycoerythrin.
Expand 2 Items
Bio-16-ddUTP, tetralithium salt
Supplier: ENZO LIFE SCIENCES
Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.
Expand 1 Items
CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates
Supplier: Biotium
CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.
Expand 11 Items
ExoBrite™ True EV Membrane Stains
Supplier: Biotium
Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.
Expand 2 Items
CF® Dye cAMP
Supplier: Biotium
Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.
Expand 2 Items
Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.
Expand 3 Items
Fluorescein-12-ddUTP
Supplier: ENZO LIFE SCIENCES
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
Expand 1 Items
HRP-conjugate stabiliser, SeramunStab® STA2
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.
Expand 1 Items
Organelle-ID™ RGB reagent II
Supplier: ENZO LIFE SCIENCES
Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids