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You searched for: Systèmes de liaison et de marquage des protéines

Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Helixyte™ iFluor® 647 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 647 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 647 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ xtra Rapid Cy5 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid Cy5 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

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Controlled Protein-Protein Crosslinking Kit, Pierce™

Controlled Protein-Protein Crosslinking Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

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ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Streptavidin, maleimide-activated

Streptavidin, maleimide-activated

Supplier: Thermo Fisher Scientific

Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.

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Antibody labelling kits, Mix-n-Stain™ Digoxigenin

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.

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Ghost Dye™ Red 710

Ghost Dye™ Red 710

Supplier: Tonbo Biosciences

Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.

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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Biotin-NHS

Supplier: Merck Millipore (Oncogene)

Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

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FluorSave™ reagent, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.

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Immunostainer enhancer, Pierce™

Immunostainer enhancer, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

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VeriFluor™ Far-Red Passive Reference Dye

Supplier: Biotium

A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.

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Bilirubin conjugate, ditaurate, disodium salt, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Bilirubin conjugate, ditaurate is suitable as a direct bilirubin standard. It behaves similarly to purified bilirubin glucuronides from human bile in diazo tests.

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AZOCOLL™ substrate, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.

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Biotinylated heparin, porcine intestinal mucosa, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Biotinylated heparin contains 5 − 8 mole-percent biotin. Biotin attachment is through a 12-atom spacer arm containing a cleavable disulfide group.

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Antibody labelling kits, Mix-n-Stain™ DNP

Supplier: Biotium

Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.

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Fluor-free acids, DyLight™

Fluor-free acids, DyLight™

Supplier: Thermo Fisher Scientific

DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

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Peptide coupling kits, HOOK™

Peptide coupling kits, HOOK™

Supplier: G-Biosciences

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.

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Biotin kits, HOOK™

Biotin kits, HOOK™

Supplier: G-Biosciences

HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.

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Biotin NTA

Biotin NTA

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

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Fluorescent dyes, M series, streptavidin conjugate

Fluorescent dyes, M series, streptavidin conjugate

Supplier: Abnova

Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.

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Protein labelling Kit, Amersham™ QuickStain

Protein labelling Kit, Amersham™ QuickStain

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

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Colorants mono-réactifs Amersham Cy3 et Cy5, paquets de 5, Cytiva

Colorants mono-réactifs Amersham Cy3 et Cy5, paquets de 5, Cytiva

Supplier: Cytiva

Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Nucléotides fluorescents, Amersham™ CyDye™

Nucléotides fluorescents, Amersham™ CyDye™

Supplier: Cytiva

Pour étiquetage des sondes d'ADN et d'ARN, notamment pour les analyses multicolores sur des microéchantillons, FISH, identification chromosomique, peinture chromosomique, établissement de caryotype et cartographie génétique.

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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Supplier: Cytiva

Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.

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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

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Reactive Dye 5-packs, Amersham CyDye™

Reactive Dye 5-packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.

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