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Protéines expression

Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.

TriLink® CleanCap® OVA mRNA

TriLink® CleanCap® OVA mRNA

Supplier: TriLink BioTechnologies

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)

TriLink® CleanCap® M6 EPO mRNA (N1MePsU)

Supplier: TriLink BioTechnologies

EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

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Autoinduction Systems, Overnight Express™ Instant LB and TB Media

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

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Freund's incomplete adjuvant, Imject™

Freund's incomplete adjuvant, Imject™

Supplier: Thermo Fisher Scientific

Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

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Alum adjuvant, AlumVax hydroxide

Alum adjuvant, AlumVax hydroxide

Supplier: OZ BIOSCIENCES

AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.

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ProLite™ His-Tag Protein Gel Staining Kit

ProLite™ His-Tag Protein Gel Staining Kit

Supplier: AAT BIOQUEST

This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.

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Alum adjuvant, AlumVax phosphate

Alum adjuvant, AlumVax phosphate

Supplier: OZ BIOSCIENCES

AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.

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Freund's incomplete adjuvant, IFAVax

Freund's incomplete adjuvant, IFAVax

Supplier: OZ BIOSCIENCES

Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).

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Autoinduction Systems, Overnight Express™ Autoinduction System 2

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

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Autoinduction systems, Overnight Express™ NMR Media

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

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Squalene emulsion, SqualVax

Squalene emulsion, SqualVax

Supplier: OZ BIOSCIENCES

SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.

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Autoinduction Systems, Overnight Express™ Autoinduction System 1

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

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Freund's complete adjuvant, Imject™

Freund's complete adjuvant, Imject™

Supplier: Thermo Fisher Scientific

Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.

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Rapid mouse antibody isotyping kit, Pierce™

Rapid mouse antibody isotyping kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.

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Freund's complete adjuvant, MP Biomedicals

Supplier: MP Biomedicals

Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of Mycobacterium is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.

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Freund's incomplete adjuvant, MP Biomedicals

Supplier: MP Biomedicals

Freund's incomplete adjuvant mixture contains mineral oil and Arlacel A.

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Système de translation rapide (RTS), kits haut rendement E. coli

Système de translation rapide (RTS), kits haut rendement E. coli

Supplier: biotechrabbit

The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.

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Système de translation rapide (RTS), kits de membranes d'insectes

Système de translation rapide (RTS), kits de membranes d'insectes

Supplier: biotechrabbit

Kit de membranes d'insectes RTS 100 pour des rendements élevés de protéines eucaryotes modifiées et synthétisées in vitro post-translation.

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Système de translation rapide (RTS), kits Germe de blé

Système de translation rapide (RTS), kits Germe de blé

Supplier: biotechrabbit

Le système de translation rapide (RTS) est une plateforme évolutive d'expression des protéines in vitro qui produit de grandes quantités de protéines pour les études de caractérisation, les essais fonctionnels ou l'analyse structurelle. Le RTS permet à la fois la transcription et la translation en utilisant un échange haut rendement (HY) et continu, des technologies sans cellule (CECF), avec des systèmes basés sur des lysats de cellules bactériennes (E. coli) ou eucaryotes (germe de blé). Le RTS offre les avantages d'un système d'expression des protéines sans cellule, tout en éliminant le recours à des étapes laborieuses et en aval généralement associées aux systèmes d'expression basés sur des cellules.

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Système de translation rapide (RTS), kits de disulfide E. coli et Fab

Système de translation rapide (RTS), kits de disulfide E. coli et Fab

Supplier: biotechrabbit

Le système de translation rapide (RTS) est une plateforme évolutive d'expression des protéines in vitro qui produit de grandes quantités de protéines pour les études de caractérisation, les essais fonctionnels ou l'analyse structurelle. Le RTS permet à la fois la transcription et la translation en utilisant un échange haut rendement (HY) et continu, des technologies sans cellule (CECF), avec des systèmes basés sur des lysats de cellules bactériennes (E. coli) ou eucaryotes (germe de blé). Le RTS offre les avantages d'un système d'expression des protéines sans cellule, tout en éliminant le recours à des étapes laborieuses et en aval généralement associées aux systèmes d'expression basés sur des cellules.

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Rapid Translation System (RTS) modèles linéaires

Rapid Translation System (RTS) modèles linéaires

Supplier: biotechrabbit

Kits de génération de modèles linéaires à base de PCR.

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