Tampons

Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.

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Tampon, TRIS, solution 1,0 M, pH 9,0

Supplier: Thermo Fisher Scientific

TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid

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SSC Solution tampon saline 20x concentrée (sodium citrate)

Supplier: LONZA

Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)

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Enzyme storage buffer in PBS and glycerol, autoclaved

Supplier: Thermo Fisher Scientific

Enzyme Storage Buffer in PBS and Glycerol

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ADA, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

N-(2-Acetimido)iminodiacetic acid monosodium salt (ADA) buffer solutions PH values 6,0 to 7,5.

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Phosphate-buffered saline (PBS, 10X), no potassium

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), No potassium, 10X, Liquid

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IEF Sample buffer (4X) pH 3 to 7 with lysine

Supplier: Thermo Fisher Scientific

Loading Buffer, IEF Sample Buffer (4X) with Lysine

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Phosphate-buffered saline (PBS, 10X), pH 7,4

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), 10X, Valeur pH: 7,4, Colourless, Liquid

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, Valeur pH: 5,00, ±0.02, Ampoule

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.