Tampons

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: Thermo Fisher Scientific

Tampon de migration, IEF Anode buffer (50X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS) with EDTA, 5X, Liquid

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, Valeur pH: 3,0, Odourless, Liquid

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 1,0% in PBS (20X)

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Corning

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)