Vous avez recherché : Isothermal Amplification Reagents

Supplier: Cytiva

The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products. It contains all of the components necessary for genomic DNA preparation by isothermal strand displacement amplification. The product protocol is very simple, and provides typical yields of 40 to 50 μg DNA and average product lengths 10 kb. The starting material for GenomiPhi reactions can be purified DNA or non purified cell lysates.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.

Supplier: Cytiva

Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimised formulation to amplify genomic DNA from 1 to 1000 cells without background interference.

Supplier: Cytiva

illustra™ TempliPhi large construct DNA amplification kit (TempliPhi LC kit) is used to prepare templates for BAC or fosmid DNA sequencing.

Supplier: Cytiva

Le kit d'amplification d'ADN illustra™ GenomiPhi™ V2, de la famille des ADN polymérases Phi29, est une préparation à petite échelle de l'ADN génomique. Le protocole est simple. Un rendement type d'ADN de 4 à 7 µg peut être obtenu en moins de deux heures avec un temps de manipulation limité. La longueur moyenne du produit est de >10 kb. La substance de départ pour les réactions GenomiPhi peut être de l'ADN purifié de n'importe quel kit commercial ou méthode particulière, ou des lysats cellulaires non purifiés.

Supplier: Cytiva

Le kit d'amplification de l'ADN illustra™ Ready-To-Go™ GenomiPhi™ HY offre une amplification d'une grande efficacité et représentative de l'ensemble du génome avec un rendement de 40 à 60 μg à partir de quelques nanogrammes d'échantillon d'ADN.

Supplier: Cytiva

For the amplification of difficult templates for successful DNA sequencing in 18 hours.