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Vous avez recherché : Milieux de culture cellulaire

Milieux de culture cellulaire

Cell culture media maintains the right pH and supports the growth of many types of cells. The media formulation includes amino acids, vitamins, glucose, inorganic salts and other growth factors designed to support the growth of cells or microorganisms

VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)

VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)

Supplier: VWR Chemicals

Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

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VWR®, Cell culture media, Schneider's Drosophila medium

VWR®, Cell culture media, Schneider's Drosophila medium

Supplier: VWR Chemicals

Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

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VWR®, Lymphocyte separation media

VWR®, Lymphocyte separation media

Supplier: VWR Chemicals

Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

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VWR®, Cell culture media, RPMI 1640

VWR®, Cell culture media, RPMI 1640

Supplier: VWR Chemicals

RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

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VWR®, Cell culture media, Minimum Essential Medium (MEM)

VWR®, Cell culture media, Minimum Essential Medium (MEM)

Supplier: VWR Chemicals

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

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VWR®, Bovine serum albumin (BSA)

VWR®, Bovine serum albumin (BSA)

Supplier: VWR Chemicals

VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.

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VWR®, Cell culture media, Ham's F12

VWR®, Cell culture media, Ham's F12

Supplier: VWR Chemicals

Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.

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VWR®, Cell culture media, McCoy's 5A medium

VWR®, Cell culture media, McCoy's 5A medium

Supplier: VWR Chemicals

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

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VWR®, L-Glutamine

VWR®, L-Glutamine

Supplier: VWR Chemicals

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.

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VWR®, Penicillin-streptomycin solution (100X)

VWR®, Penicillin-streptomycin solution (100X)

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

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VWR®, Cell culture media, Medium 199

VWR®, Cell culture media, Medium 199

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

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Murashige and skoog modified basal salts (without KH₂PO₄)

Supplier: Apollo Scientific

Contains macro/micronutrients, sucrose, agar and organics as described by Murashige and Skoog, 1962.Plant tissue culture testedFull specification given in appendix.

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Murashige and skoog basal mixture

Supplier: Apollo Scientific

Murashige and skoog basal mixture 10 L

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Fibroblast Growth Medium, Basal

Fibroblast Growth Medium, Basal

Supplier: PROVITRO

Fibroblast Growth Medium is a sterile liquid culture medium designed for cultivating human fibroblasts (HFIB). Provided as a basal medium, it is ideal for culturing Provitro HFIB upon the addition of supplement mix components.

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DMEM Low Glucose with Stable Glutamine with Sodium Pyruvate

Supplier: Biowest

Dulbecco's Modified Eagle medium (DMEM) is formulated to preserve and maintain the growth of a wide range of mammalian cells. Supplemented with fetal calf serum, DMEM also allows the proliferation of mouse embryonic stem cells.

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Cell culture medium, ActiSM, HyClone™

Cell culture medium, ActiSM, HyClone™

Supplier: HyClone products (Cytiva)

HyClone™ ActiSM cell culture media is a chemically defined animal-derived component-free (ADCF) cultured media optimised for high-yield protein production for bioprocessing. This media is designed for use with HyClone™ ActiPro cell culture media.

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HyClone™ VaccineXpress Cell Culture Media

HyClone™ VaccineXpress Cell Culture Media

Supplier: HyClone products (Cytiva)

HyClone VaccineXpress cell culture medium is designed and developed for high‑density growth and maintenance of kidney‑derived cell lines (e.g., Vero cells) for viral vaccine development. VaccineXpress medium is well suited for use in multi-well plates and large‑scale manufacturing using microcarriers in WAVE Bioreactor or Xcellerex bioreactors.

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Orchid seed sowing medium

Supplier: Apollo Scientific

Mother flasking medium II.

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hMSC Chondrogenesis Induction Medium

hMSC Chondrogenesis Induction Medium

Supplier: PROVITRO

The hMSC Chondrogenesis Induction Medium is a sterile liquid culture medium specifically designed for initiating chondrogenic differentiation in human mesenchymal stem cells (hMSCs). This medium is provided as a basal formulation and becomes fully functional for hMSC culture upon the addition of all the required supplement mix components.

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Murashige and skoog modified basal medium (with 2iP And IAA)

Supplier: Apollo Scientific

Contains macro/micronutrients as described by Murashige and Skoog, 1962. Modified with addition of 2iP, Adenine hemisulphate and IAA. Plant tissue culture testedFull specification given in appendix.

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Quoirin and Lepoivre basal salt mixture

Supplier: Apollo Scientific

Quoirin and Lepoivre basal salt mixture 10×1 L

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Cell culture media, HyClone™ ActiPro™

Cell culture media, HyClone™ ActiPro™

Supplier: HyClone products (Cytiva)

HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.

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Cell culture media, Glasgow MEM (GMEM BHK 21)

Cell culture media, Glasgow MEM (GMEM BHK 21)

Supplier: Biowest

Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

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Orchid replate medium

Supplier: Apollo Scientific

Proprietary formulation.

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Urothelial Cell Growth Medium

Urothelial Cell Growth Medium

Supplier: PROVITRO

Urothelial Cell Growth Medium is a sterile, serum-free liquid culture medium designed for the cultivation of Human Urothelial Epithelial Cells (HUEPC). Delivered in the form of a basal medium, it becomes a complete and suitable environment for culturing HUEPC upon the addition of three supplement components.

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Malmgren modified terrestrial orchid medium

Supplier: Apollo Scientific

Malmgren modified terrestrial orchid medium 10×1 L

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Insect cell culture media, serum-free, CCM3, HyClone™

Insect cell culture media, serum-free, CCM3, HyClone™

Supplier: HyClone products (Cytiva)

HyClone™ CCM3 media is designed to increase cell yields with multiple lines in a variety of culture environments. It is tailored for Sf9 cells.

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HyClone CDM4PERMAb

HyClone CDM4PERMAb

Supplier: HyClone products (Cytiva)

HyClone™ CDM4PERMAb is a high-performance cell culture medium developed to increase process yields in the production of human antibodies and recombinant proteins using PER.C6 (Crucell NV) technology. Manufactured according to cGMP guidelines.

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Nitsch and Nitsch basal salt mixture

Supplier: Apollo Scientific

With the macro/micronutrients as described by Nitsch and Nitsch, 1969 Plant tissue culture tested Full specification given in appendix.

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Heller's basal salt mixture

Supplier: Apollo Scientific

Contains macro/micronutrients as described by Heller, 1953 Plant tissue culture testedFull specification given in appendix.

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