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Réactifs de transfection

VWR offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9, and more. When considering a suitable transfection reagent, it is important to identify the cell type and culture conditions. Specialised transfection reagents will address rare cell cultures, neurons, and primary cells are more difficult to manipulate for porous characteristics. VWR also offers products for electroporation and the consumables needed to support the transfection workflow.

Transduction enhancer, AdenoMag™

Transduction enhancer, AdenoMag™

Supplier: OZ BIOSCIENCES

AdenoMag™ transfection reagent is a magnetic nanoparticles formulation specifically dedicated to enhance Adenovirus and Adeno Associated Virus (AAV) infection and transduction capacities. Based on Magnetofection™ technology, it allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses.

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Transduction enhancer, in vivo ViroMag™

Transduction enhancer, in vivo ViroMag™

Supplier: OZ BIOSCIENCES

In vivo ViroMag™ transfection reagent has been designed for in vivo targeted transduction with any viral vector. Virus/nanoparticle complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

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Transduction reagent, Viro-MICST™

Transduction reagent, Viro-MICST™

Supplier: OZ BIOSCIENCES

Viro-MICST™ transduction reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on i-MICST™ Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection/Transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system. For efficient and selective gene delivery to target cells, Viro-MICST™ can be used in combination with any viral vector and allows you to reduce cell manipulation steps and save time and material.

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Transduction reagent, ViroMag™ R/L

Transduction reagent, ViroMag™ R/L

Supplier: OZ BIOSCIENCES

ViroMag™ R/L transduction reagent is a magnetic nanoparticles formulation optimised for rRetroviruses and lentiviruses. Based on Magnetofection™ technology, this reagent allows concentrating the complete applied dose of retro/lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses.

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Transduction reagent, ViroMag™

Supplier: OZ BIOSCIENCES

ViroMag™ transduction reagent is a magnetic nanoparticles formulation dedicated to increase virus infection and transduction capacities. Based on Magnetofection™ technology, this reagent allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus transduction with extremely low vector doses. Due to its specific properties, ViroMag™ is ideal to infect non permissive cells.

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Réactif de transfection, Insect GeneJuice®

Supplier: Merck Millipore (Novagen)

Le réactif de transfection Insect GeneJuice® est formulé pour optimiser l'efficacité de la transfection des cellules d'insectes Sf9. Le réactif apporte une toxicité extrêmement faible aux cellules et peut être utilisé pour les transfections transitoires et stables dans les milieux avec ou sans sérum, et pour la cotransfection des plasmides de transfert avec ADN de virus linéarisé pour la production de baculovirus recombinés.

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Bipolar transfection system, HBIO AgilePulse PLUS

Bipolar transfection system, HBIO AgilePulse PLUS

Supplier: BTX

Easily perform advanced transfection protocols with multiple groups of pulses-each with separate parameters.

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Protein transfection reagent, Novagen®, ProteoJuice™

Supplier: Merck Millipore (Novagen)

ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

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siRNA transfection reagent, RiboJuice™

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

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Transfection reagent, 293-Free™

Supplier: Merck Millipore (Novagen)

293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

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Transfection kit, BacMagic™-2

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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Transfection kit, BacMagic™

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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DNA kit, BacMagic™-2

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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Mélange de dNTP (10 mM)

Mélange de dNTP (10 mM)

Supplier: STEMCELL Technologies

Solution contenant des sels de sodium de dATP, dCTP, dGTP et dTTP, chacun à 10 mM.

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Kit ArciTect™ T7 Endonucléase I

Kit ArciTect™ T7 Endonucléase I

Supplier: STEMCELL Technologies

Pour l'estimation de l'efficacité de l'édition du génome par CRISPR-Cas9.

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ArciTect™ Human HPRT Positive Control Kit

ArciTect™ Human HPRT Positive Control Kit

Supplier: STEMCELL Technologies

Positive control for CRISPR-Cas9 genome editing.

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ArciTect™ crRNA

ArciTect™ crRNA

Supplier: STEMCELL Technologies

ARN CRISPR conçu sur mesure pour la génération d'ARN guide dans l'édition du génome CRISPR-Cas9.

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ArciTect™ tracrRNA Kit

ArciTect™ tracrRNA Kit

Supplier: STEMCELL Technologies

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.

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ArciTect™ Annealing Buffer (5X)

ArciTect™ Annealing Buffer (5X)

Supplier: STEMCELL Technologies

For guide RNA generation in CRISPR-Cas9 genome editing.

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Transfection reagents, DharmaFECT® siRNA

Supplier: HORIZON DISCOVERY

A panel of four transfection reagents optimised for the efficient delivery of siRNA into mammalian cells.

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STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

Supplier: STEMCELL Technologies

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.

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STEMscript™ kit de synthèse d'ADNc avec amorces aléatoires

STEMscript™ kit de synthèse d'ADNc avec amorces aléatoires

Supplier: STEMCELL Technologies

Pour la transcription inverse d'ARN sans queue poly(A), avant qPCR ou séquençage d'ARN.

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Nucléase ArciTect™ Cas9

Nucléase ArciTect™ Cas9

Supplier: STEMCELL Technologies

Nucléase Cas9 (4 μg/μl) pour la génération de cassures double-brin dans l'édition du génome par CRISPR-Cas9.

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ArciTect™ Cas9-eGFP Nuclease

ArciTect™ Cas9-eGFP Nuclease

Supplier: STEMCELL Technologies

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing.

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ArciTect™ High-Fidelity DNA Polymerase Kit

ArciTect™ High-Fidelity DNA Polymerase Kit

Supplier: STEMCELL Technologies

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification.

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