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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
Lysis buffer, CTAB
Supplier: PanReac AppliChem
Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.
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RIPA lysis and extraction buffer, Pierce™
Supplier: Thermo Fisher Scientific
The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
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I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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IP lysis buffer, Pierce™
Supplier: Thermo Fisher Scientific
The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.
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Protein solubilisation buffer
Supplier: G-Biosciences
FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
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Luciferase cell lysis buffer (2X), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Nuclear and cytoplasmic extraction reagents, NE-PER
Supplier: Thermo Fisher Scientific
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
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Mammalian protein extract reagents, M-PER™
Supplier: Thermo Fisher Scientific
The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
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Extraction buffers, FOCUS™
Supplier: G-Biosciences
FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.
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Réactif d'extraction de protéines, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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Réactif d'extraction de protéines, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
Le réactif d'extraction de protéines BugBuster HT associe le réactif d'extraction de protéines BugBuster et la nucléase Benzonase® dans un réactif pratique. Le réactif BugBuster HT écarte les problèmes de biotraitement courants dus aux procédures traditionnelles de lyse des cellules. Les protéines solubles sont extraites en douceur de l'E. coli, sans exposition à la chaleur ni stress oxydatif, et la viscosité est éliminée par la digestion des acides nucléiques en une seule étape. L'extrait de protéines qui en résulte peut être facilement fractionné par les techniques de purification classiques. BugBuster HT est parfaitement adapté aux applications de purification des protéines à haut rendement.
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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Réactif d'extraction de protéines, BugBuster® (sans amine primaire), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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MES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific
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ACK Lysing Buffer (1x), TheraPEAK™
Supplier: LONZA
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.
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Succinate, 0,2 M buffer solution, pH 5,0
Supplier: Thermo Fisher Scientific
Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific
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NP-40 lysis buffer (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer (2X), Thermo Fisher Scientific
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HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Supplier: GRANT INSTRUMENTS
Supplier: BOCHEM
Supplier: VWR Collection
Supplier: OMEGA BIO-TEK
Supplier: DWK Life Sciences
Supplier: Thermo Fisher Scientific
Supplier: VWR Collection
Supplier: Oxford Nanopore Technologies
Supplier: Industrial Physics
Supplier: AAT Bioquest
Supplier: Integra
Supplier: Palbam
Supplier: VWR Collection
Supplier: Integra
Supplier: VWR Collection
Supplier: FACELLITATE
Supplier: NANOENTEK
Supplier: Greiner Bio-One