Tampons d'extraction
Les tampons d’extraction sont prêts à l’emploi et efficaces pour l’isolement de l’ARN total et d’autres protéines, améliorant le rendement protéique et la complexité des échantillons à partir de cellules et de tissus. Des réactifs d’extraction optimisés favorisent l’extraction des protéines dans des tampons natifs ou dénaturants compatibles avec les techniques d’analyse en aval. Une solution tampon appropriée ajoutée à un mélange de protéines pendant le processus d’extraction peut améliorer la stabilité des molécules protéiques lorsqu’elles sont soumises à diverses forces visant à les isoler pour étude.
Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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NucleoSpin® Kits for Genomic DNA Extraction
Supplier: MACHEREY-NAGEL
Combining selective purification technologies and chemistries, these are a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT Bioquest
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Réactif d'extraction de protéines, BugBuster® (sans amine primaire), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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Réactifs d'extraction de protéines, BugBuster® et BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)
Le réactif d'extraction de protéines BugBuster® est formulé de façon à rompre délicatement la paroi cellulaire de l'E. coli, afin de libérer la protéine soluble. Il offre une alternative simple, rapide et économique aux méthodes mécaniques (French Press ou sonication) d'extraction de protéines cibles à des fins de purification ou d'autres applications. La formulation utilise un mélange de détergents capable de perforer la paroi cellulaire sans dénaturer la protéine soluble.
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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RBC lysis buffer for human
Supplier: Thermo Fisher Scientific
RBC lysis buffer for human, Thermo Fisher Scientific
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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