Tampons d'extraction

Les tampons d’extraction sont prêts à l’emploi et efficaces pour l’isolement de l’ARN total et d’autres protéines, améliorant le rendement protéique et la complexité des échantillons à partir de cellules et de tissus. Des réactifs d’extraction optimisés favorisent l’extraction des protéines dans des tampons natifs ou dénaturants compatibles avec les techniques d’analyse en aval. Une solution tampon appropriée ajoutée à un mélange de protéines pendant le processus d’extraction peut améliorer la stabilité des molécules protéiques lorsqu’elles sont soumises à diverses forces visant à les isoler pour étude.

Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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RIPA cell lysis buffer 2

Supplier: Enzo Life Sciences

RIPA cell lysis buffer 2, Enzo Life Sciences

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1X Red Blood Cell Lysis Buffer, Abcam

Supplier: Abcam

Red Blood Cell (RBC) Lysis Buffer ab286858 provides a quick and efficient method of lysing red blood cells. It is provided in a 1x concentrated format, and is also available as ab204733 in a 10x format.

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Extraction reagents

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.

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Guanidine hydrochloride buffer solution, 6 mol/L, pH 8,7

Supplier: TCI

Guanidine Hydrochloride Buffer Solution, 6 mol/L, Valeur pH: 8,7, TCI

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Réactif stabilisateur E.Z.N.A.® RNALock

Supplier: OMEGA BIO-TEK

Le réactif stabilisateur E.Z.N.A.® RNALock est un réactif innovant qui lyse rapidement les cellules et les tissus pour désactiver les RNases à température ambiante. Il suffit d'immerger les échantillons, tels que les cultures cellulaires, les tissus animaux ou les globules blancs, dans le réactif stabilisateur RNALock. L'ARN cellulaire intact sera préservé même à température élevée. Les échantillons peuvent être conservés dans ce réactif pendant plus de 12 mois à -20 °C.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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ReadiUse™ Viral RNA Lysis Buffer

Supplier: AAT Bioquest

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

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Protein extraction kit, mammalian cell PE LB™

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

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Protein extraction kit, Bacterial PE LB™

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

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Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

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Protein extraction kit, tissue PE LB™

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.