Tampons d'hybridation

Les tampons d’électrophorèse maintiennent un pH constant lors de la séparation de l’ADN, de l’ARN et d’autres protéines. En fournissant les ions nécessaires au transfert du courant appliqué, ces solutions faiblement chargées permettent aux molécules de différentes tailles de migrer à des vitesses variables pour l’analyse. Offrant une protection supplémentaire aux protéines, les substances préparatoires empêchent leur dégradation, garantissant la viabilité des échantillons et la fiabilité des résultats. Disponibles dans différentes masses moléculaires, les meilleurs tampons peuvent être choisis selon les macromolécules manipulées.

Tampon SSC, 20X, ultrapure

Supplier: VWR Chemicals

Le tampon SSC est un tampon d'hybridation pour les transferts Northern et Southern.

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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)

Supplier: G-Biosciences

Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.

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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

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In-situ hybridisation buffer for HPV probes

Supplier: Enzo Life Sciences

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

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VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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In situ hybridisation wash reagent

Supplier: Enzo Life Sciences

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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SSC Solution tampon saline 20x concentrée (sodium citrate)

Supplier: BIOMOL RESEARCH LABORATORIES

Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)

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SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences

Supplier: Enzo Life Sciences

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

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Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

Supplier: G-Biosciences

Saline-sodium phosphate-EDTA hybridisation buffer [20X].

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.