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Cellules compétentes

Cellules compétentes

Les cellules compétentes sont celles dont les parois cellulaires ont été modifiées afin de faciliter l’incorporation d’ADN étranger dans leur intérieur. Les cellules sont rendues compétentes par un processus utilisant le chlorure de calcium et un choc thermique, et les cellules en croissance très rapide deviennent compétentes plus facilement que celles à d’autres stades de croissance. Des facteurs tels que l’efficacité de transformation, les marqueurs génétiques et les formats de conditionnement influenceront le temps et l’effort requis pour votre projet, ainsi que son succès. Choisissez parmi plusieurs types et formats cellulaires afin d’assurer une fonction optimale.

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Protein expression competent cell pack

Supplier: AGILENT

Protein Expression competent cell packs efficiently test multiple strains for expression.

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SCS110 competent cells

Supplier: AGILENT

SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA.

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Competent cells, ArcticExpress

Supplier: AGILENT

Tackle two common bacterial gene expression hurdles: protein insolubility and codon bias.

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EletroTen-blue electroporation-competent cells

Supplier: AGILENT

Achieve the highest transformation efficiencies available when cloning large plasmids and ligated DNA.

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TG1 electroporation-competent cells

Supplier: AGILENT

Phage display libraries from electroporation-competent cells with high transformation efficiency.

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SCSI supercompetent cells

Supplier: AGILENT

SCSI supercompetent and DH5α competent cells are classic E. coli strains engineered for competence.

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SURE competent cells

Supplier: AGILENT

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.

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XL-1 red competent cells

Supplier: AGILENT

XL-1 Red Competent Cells are ideal for introducing random mutations into a cloned gene of interest.

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XL10-Gold ultracompetent cells

XL10-Gold ultracompetent cells

Supplier: AGILENT

XL10-Gold ultracompetent cells transform large plasmids and ligated DNA with highest efficiency.

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DH5α Chemically Competent E. coli Cells

Supplier: OriGene

Competent Cells, DH5α chemically competent cells (≥10⁸ CFU/μg of pUC19 DNA)

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XL1 blue and XL2 blue competent cells

Supplier: AGILENT

XL1 and XL2-Blue Competent Cells are versatile for routine cloning and allow blue-white colour screening for high efficiency cloning of methylated DNA.

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BL21 competent cells

Supplier: AGILENT

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

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TK competent cells

Supplier: AGILENT

Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target.

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ABLE C competent cells

Supplier: AGILENT

ABLE Competent Cells for toxic clones reduce copy number four to ten fold compared to the XL1-Blue strain.

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BJ5183 Electroporation-competent cells

Supplier: AGILENT

BJ5183 electroporation competent cells are a recombination proficient bacterial strain.

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JM competent cells

Supplier: AGILENT

JM Competent Cells are recombination- and E. coli K12 restriction-proficient.

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