Vous avez recherché : Réactifs pour la microscopie et la coloration

Supplier: VWR Chemicals

Q Path® Safesolv est un substitut non dangereux du xylène ou du toluène. Il est compatible avec le milieu de montage Q Path® Safemount référence 00647520. Il est utilisé à la place du xylène, du toluène, du méthylecyclohexane ou d'autres solvants au cours des étapes de déshydratation des préparations histologiques, ainsi que pendant les phases de coloration et de montage de lamelles.

Supplier: SIGMA ALDRICH MICROSCOPY

Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: TCI

Specifications
Appearance: Colorless to Almost colorless clear liquid
Functionality test: to pass test (Remaining fluorescence strength)

Supplier: TCI

Specifications
Appearance: Colorless to Light orange to Yellow clear liquid
Functionality test: to pass test (Remaining fluorescence strength)

Supplier: TCI

Specifications
Appearance: Colorless to Light yellow clear liquid
Functionality test (transparency) (display): to pass test

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

Supplier: TCI

Specifications
Appearance: Colorless to Light yellow to Light orange clear liquid
Decoloring test: min. 1.0 (400 to 410 nm)

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: Merck

Les produits de montage anhydre, telles que Neo-Mount®, doivent être utilisés pour le montage permanent et le stockage à long terme d'échantillons. Les lames traitées dans les laboratoires d'anatomo-pathologie devant être stockées jusqu'à 10 ans, les résultats de coloration doivent être stables et une décoloration n'est pas acceptable. Le milieu de montage anhydre Neo-Mount® est un milieu de montage pour microscopie à la coloration extrêmement stable, fabriqué avec des solvants à base de mélanges d'hydrocarbures aliphatiques. Il contient un substitut non aromatique du xylène, par conséquent, Neo-Mount® doit être combiné exclusivement avec Neo-Clear®. Le Xylène doit être évité dans l'étape de montage, car il entraîne une opacification des lames et laisse des traces. En outre, en plaçant les lames déshydratée sur papier filtre pendant environ 1 minute avant montage, tout excès de Neo-Clear® peut être évité, étant donné que des bulles d'air peuvent apparaître sous la lamelle couvre-objet. La même condition préalable doit également être respectée lors du montage des échantillons à l'aide d'une machine de montage automatique ; dans ce cadre, le NEO-CLEAR® peut être éliminé de la façon la plus efficace par incubation des lames pendant une minute dans un portoir à lames vide.

Supplier: ENZO LIFE SCIENCES

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

Supplier: TCI

Specifications
Appearance: Colorless to Almost colorless clear liquid
Functionality test: to pass test (Remaining fluorescence strength)
Decoloring test: min. 1.0 (400 to 410 nm)

Supplier: TCI

Specifications
Appearance: Colorless to Almost colorless clear liquid
Functionality test: to pass test (Remaining fluorescence strength)
Decalcification test: max. 0.3 (590 to 600 nm)

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: ENZO LIFE SCIENCES

The IHC PAP pen provides a hydrophobic barrier when a circle is drawn around a specimen on a glass slide.

Supplier: ENZO LIFE SCIENCES

Fluoromount is an aqueous-based permanent, high stability mounting medium designed for fluorogenic labelling and ideal for long-term storage of processed tissue.

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

Supplier: ENZO LIFE SCIENCES

IHC mount is an aqueous-based permanent, high stability mounting medium designed for use with chromogenic labeling. Use for mounting of tissue specimens stained with peroxidase and alkaline phosphatase-based systems and various fluorescent dyes.

Supplier: PanReac AppliChem

This fluorescence-free immersion oil for microscopy is highly UV-permeable and free of PCB's.

Supplier: AAT BIOQUEST

The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

Supplier: AAT BIOQUEST

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: PanReac AppliChem

Davidson solution is a rapid fixative.

Supplier: PanReac AppliChem

Fixative for preserving soft and delicate structures, used as a mordant in various trichrome procedures.

Supplier: AAT BIOQUEST

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: AAT BIOQUEST

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50 to 70%.

Supplier: AAT BIOQUEST

FluoroQuest™ Antifade mounting medium is a specialised and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

Supplier: AAT BIOQUEST

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: AAT BIOQUEST

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: Thermo Fisher Scientific

Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.