Vous avez recherché : Suppléments et additifs pour culture cellulaire

Supplier: Abcam

Phosphatase Inhibitor Cocktail IV (ab201115) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Enzo Life Sciences

The Screen-Well® Wnt pathway library v 2.0 is a focused collection of 71 compounds with defined and diverse Wnt pathway activity, including activators and inhibitors of Wnts, Dishevelled, GSK-3β and other Wnt pathway kinases, TCF/β-catenin, DKK, LRP, Axin, Porcupine, and more. Compounds are dissolved in DMSO at 10 mM or 1 mM and aliquoted into deep-well plates at 100 or 500 µl per well. A variety of structurally and mechanistically different compound classes are included. A useful tool for studying the roles of pro- and anti-Wnt pathway molecules in cells as well as for use in in vitro applications.

Supplier: Enzo Life Sciences

The adrenergic ligand library contains 84 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The adrenergic ligand library is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.

Supplier: Enzo Life Sciences

The Screen-Well™ Phosphatase Inhibitor Library contains 33 known phosphatase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 µl or 500 µl per well. The library is ideal for chemical genomics, assay development, and as a reference set for secondary screening. Includes inhibitors of these important phosphatases: Calcineurin(PP2B), JSP-1, PRL-1, CD45, PP1, PRL-3, CDC25, PP2A, PTEN, and many more.

Supplier: Enzo Life Sciences

The Screen-Well® neurotransmitter library contains 681 CNS receptor ligands in a 96-well format. Ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, validating new assays, and for routine pharmacological applications.

Supplier: PROVITRO

The supplement kit designed for cultivating human smooth muscle cells (HSMC) provides an optimised formulation for promoting growth and maintaining the health of these cells. Upon adding the components of the supplement kit to a 500 ml basal medium, a specialised environment is created to support the culture of HSMCs. The formulation is specifically tailored for the initial seeding of 4000 cells per square centimeter, aiming to achieve confluence at approximately 90%. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk tellurite emulsion

Supplier: Abcam

Dynamin Inhibitors Toolbox (ab120468) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Thermo Fisher Scientific

Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis. The Halt Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts.

Supplier: Charles River Laboratories Cell Solutions

HypoThermosol® biopreservation media is a novel, engineered, optimised hypothermic storage and shipping media product. Serum-free, protein-free HypoThermosol® is designed to provide maximum storage and shipping stability for biologics at 2 to 8 °C.

Supplier: STEMBIOSYS

CELLvo™ XF Matrix is a ready to use cell culture substrate which provides a xeno-free native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.

Supplier: STEMBIOSYS

CELLvo™ Matrix Plus is a substrate that aims to recapitulate the biochemical, structural and mechanical cues of the native niche by leaving the extracellular matrix intact. Cell-derived matrices create a biologically relevant culture environment for a variety of cell types including pluripotent cells by providing all the cues cells expect to receive in vivo in culture dishes.

Supplier: PanReac AppliChem

Yeast extract BioChemica

Supplier: Corning

Interleukin-3 (IL-3) is a glycoprotein that regulates differentiation and enhances proliferation of a variety of hematopoietic precursor cells, mast cells, basophils, and NK cells.

Supplier: Corning

Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.

Supplier: Corning

Corning® Nu-Serum™ IV is a growth medium supplement that provides a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture.

Supplier: STEMBIOSYS

CELLvo™ Matrix is a ready to use cell culture substrate which provides a native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.

Supplier: Corning

Le diluant pour sérum Corning® MITO+ est une formulation concentrée et pleinement définie d'hormones, de facteurs de croissance (EGF et FGF) et autres métabolites (insuline et hormones stéroïdes). Il peut être utilisé pour la culture d'une variété de cellules dans des milieux sans sérum ou à sérum réduit.

Supplier: Corning

ITS contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media.

Supplier: Thermo Fisher Scientific

These inhibitor tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Supplier: Corning

Le supplément de croissance des cellules endothéliales (ECGS) est un supplément fréquemment utilisé pour la culture d'une grande variété de cellules, notamment les cellules endothéliales. L'ECGS contient divers facteurs de croissance (par exemple, FGF acide ou ECGF-a).

Supplier: Corning

T-STIM with ConA (IL-2 culture supplement), rat, is used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes.

Supplier: Corning

L'extrait pituitaire bovin (BPE) est un supplément fréquemment utilisé pour la culture d'une variété de cellules épithéliales et endothéliales. Le BPE contient des hormones et des facteurs de croissance (par exemple, FGF basique).

Supplier: Corning

Système in vitro pour l'évaluation du potentiel invasif des cellules malignes et normales.

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

Cells grown in traditional media supplemented with L-glutamine are susceptible to toxicity.

Supplier: Thermo Fisher Scientific

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.