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731 results for "buffers"

You searched for: buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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TISAB I Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Cesium nitrate, ionization buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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HDAC assay buffer

Supplier: ENZO LIFE SCIENCES

HDAC assay buffer

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: G-Biosciences

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Gram Negative lysis buffer

Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Mini flip-top vial with 9 ml Butterfield's buffer

Mini flip-top vial with 9 ml Butterfield's buffer

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Butterfield's Buffer is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations

VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations

Supplier: VWR Chemicals

Total ionic strength adjustment buffer solution for use with selective ion electrodes.

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Protein Transfer buffers

Supplier: G-Biosciences

Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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VWR®, pH standard kit

VWR®, pH standard kit

Supplier: VWR Chemicals

Kit contains 500 ml of pH 4,0, pH 7,0 and pH 10,0 standards.

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Caspase cell lysis buffer

Supplier: ENZO LIFE SCIENCES

Caspase cell lysis buffer, Enzo Life Sciences

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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

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MMP-1 colorimetric assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 colorimetric assay buffer

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

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Diluent buffer, 10X concentrate, for dab membrane substrate

Supplier: Rockland Immunochemicals

Concentrate diluent buffer for DAB membrane substrate, 10X

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RBC Lysis Buffer (10X)

RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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Cell lysis buffer, RIPA

Supplier: ENZO LIFE SCIENCES

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Supplier: Rockland Immunochemicals

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

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Hybridisation buffers

Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT™ dissolution media concentrate, sodium phosphate, 961,5 ml, J.T.Baker® DILUT-IT®, pH value: 6,8

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In-situ hybridisation buffer for HPV probes

Supplier: ENZO LIFE SCIENCES

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

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Competent cell buffer

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

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