buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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Tris-EDTA (TE) buffer solution for molecular biology, pH 7,4, BioUltra, Supelco®

Supplier: Merck

Tris-EDTA buffer solution has been used for the dilution of DNA samples.[1] It has been used for the elution of purified vector.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

Supplier: G-Biosciences

Saline-sodium phosphate-EDTA hybridisation buffer [20X].

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RIPA Buffer, Abcam

Supplier: Abcam

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.

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VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure

Supplier: VWR Chemicals

Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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EDTA Buffer, pH 8,5, 10X, Antigen Retriever

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Blocking Buffer 10X

Supplier: Abcam

Blocking Buffer 10X is part of the reagents, controls and accessories range.

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10X Phosphate Buffered Saline, Abcam

Supplier: Abcam

10X Phosphate Buffered Saline is a buffer designed for ELISA assay development.

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Kinase Assay Buffer I, Abcam

Supplier: Abcam

Stock solution of buffer used for assaying kinase protein.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.