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"Staining Solutions"

 
 

Calcein fluorescent indicator

Supplier: Cayman Chemical

Calcein is a xanthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. Excitation and emission maxima for calcein are 494 and 517 nm, respectively.

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Technical pH buffer solutions in sachets, HI 50000 series

Supplier: Hanna

These technical calibration buffers produced in accordance with ISO 3696/BS3978 standards using high purity salts, deionised water, certified weight-checked balances, and Class A glassware in a temperature controlled environment monitored with certified thermometers.

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Propidium iodide 10 mM in aqueous solution ≥95%

Propidium iodide 10 mM in aqueous solution ≥95%

Supplier: AAT Bioquest

Propidium iodide (PI) belongs to the same chemical class of ethidium bromide.

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Nuclear Violet™ DCS1 5 mM in DMSO

Supplier: AAT Bioquest

Our Nuclear Violet™ DCS1 is a fluorogenic, DNA-selective and live cell-impermeant dye for analyzing DNA content in dead or fixed cells.

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Lipofuscin autofluorescence quencher, TrueBlack™

Supplier: Biotium

TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.

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Anti-ZNFN1A2/HELIOS 22F6 Monoclonal Antibody [clone: 22F6]

Supplier: Antibodies.com

Armenian hamster monoclonal [22F6] antibody to ZNFN1A2/HELIOS for Flow Cytometry with samples derived from Mouse and Human.

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Nuclear Red™ DCS1

Nuclear Red™ DCS1

Supplier: AAT Bioquest

Our Nuclear Red™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analysing DNA content in dead, fixed or apoptotic cells.

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Nuclear Blue™ DCS1

Nuclear Blue™ DCS1

Supplier: AAT Bioquest

Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells.

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Nuclear Orange™ DCS1

Nuclear Orange™ DCS1

Supplier: AAT Bioquest

Our Nuclear Orange™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells.

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Propidium Iodide solution 1 mg/ml in deionised water

Supplier: G-Biosciences

A membrane-impermeable and nucleic acid intercalator red fluorescent dye - Propidium iodide (PI).

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Nuclear Green™ DCS1

Nuclear Green™ DCS1

Supplier: AAT Bioquest

Our Nuclear Green™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analysing DNA content in dead, fixed or apoptotic cells.

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Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labelling

Supplier: Sartorius

Incucyte® Nuclight rapid red dye for live-cell nuclear labelling was formulated explicitly for cell labelling of living mammalian cells. The dye is a cell-permeable DNA strain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantitation.

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Lobby Pro® Dustpans and Broom

Lobby Pro® Dustpans and Broom

Supplier: Rubbermaid Commercial Products

A range of pans and a matching brooms for cleaning in any environment.

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STAINING SOLUTION 1 * 2 L

Supplier: SARSTEDT

STAINING SOLUTION 1 * 2 L

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STAINING SOLUTION 1 * 3 L

Supplier: SARSTEDT

STAINING SOLUTION 1 * 3 L

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3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ≥98%, yellow powder cell culture reagent

Supplier: MP Biomedicals

Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

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