Supplier: SIGMA ALDRICH MICROSCOPY

Rose bengal,certified by the Biological Stain Commission, Dye content ≥80 % (4,5,6,7-Tetrachloro-2',4',5',7'-tetraiodofluorescein disodium salt; Acid Red 94; Bengal Rose B sodium salt; Rose Bengal sodium salt).

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein 95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Supplier: SIGMA ALDRICH MICROSCOPY

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity. One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Supplier: SIGMA ALDRICH MICROSCOPY

Haematoxylin

Supplier: SIGMA ALDRICH MICROSCOPY

Inhibits mitochondrial Ca2+ uniporter; capsaicin antagonist; blocks VR1 vanilloid receptor-coupled ion channels.

Supplier: SIGMA ALDRICH MICROSCOPY

Azure B eosinate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1,2,3,3-Tetramethyl-3H-indolium iodide,98%

Supplier: SIGMA ALDRICH MICROSCOPY

Pararosaniline acetate, Dye content 90% (Basic Parafuchsin; Basic Red 9; Magenta(TM) O).

Supplier: SIGMA ALDRICH MICROSCOPY

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

Supplier: SIGMA ALDRICH MICROSCOPY

4-Nitroguaiacol potassium salt hydrate,98% (2-Methoxy-4-nitrophenol potassium salt)

Supplier: SIGMA ALDRICH MICROSCOPY

May contain minor quantities of precipitated dye.

Supplier: SIGMA ALDRICH MICROSCOPY

A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

Supplier: SIGMA ALDRICH MICROSCOPY

Sulforhodamine 101 acid chloride has been used in an encapsulated fluorescent probe as a model drug to study drug-delivery properties of rhamnogalacturonan-I based microcapsules. It has also been used in confocal imaging as an astrocyte specific dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Kit component in the silver stain kit.

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a kit component in Gram Stain Procedure (Sigma Procedure HT90). Iodine, 0.33%, and potassium iodide, 0.66%.

Supplier: SIGMA ALDRICH MICROSCOPY

Fast Dark Blue R Salt, (Azoic Diazo Component 51; Azoic Diazo No. 51)

Supplier: SIGMA ALDRICH MICROSCOPY

5,10,15,20-Tetrakis(4-methoxyphenyl)-21H,23H-porphine is an achiral porphyrin derivative. It forms chiral supramolecular assemblies via spontaneous symmetry breaking at the air or water interface.

Supplier: SIGMA ALDRICH MICROSCOPY

Vanillin azine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Erythrosin yellowish, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable as a laser dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: SIGMA ALDRICH MICROSCOPY

Amine-reactive haptenylation reagent. This succinimidyl ester derivative of digoxigenin has been shown to inhibit the Na+/K+- ATPase by binding to the cardiac steroid receptor site.

Supplier: SIGMA ALDRICH MICROSCOPY

Leucoberbelin blue I has been used as a quencher in time-resolved fluorescence QRET technique for receptor-ligand interaction screening and to detect oxidized manganese species in Mn(II) (manganese) oxidation assays.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol blue sodium salt 0.04% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers and matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.