1051 Results for: "SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs). Acridine orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine orange dye has been used to analyse mitochondria and lysosomal content by flow cytometry, characterise multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

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Hydroxy naphthol blue disodium salt, ACS reagent.

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Popular hematology stain used for differentially staining the cellular elements of blood. For dip, rack, and batch staining techniques.

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine B is a thiol-reactive and photostable fluorescent dye. Rhodamine B isothiocyanate is an aminoxanthene that possesses carboxylic acid and isothiocyanate substituents on the pendant phenyl ring.

Supplier: SIGMA ALDRICH MICROSCOPY

Ethidium bromide monoazide. ≥95% (HPLC), solid (3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide; Ethidium monoazide bromide)

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N,N'-Bis(salicylidene)-1,2-phenylenediamine,97%

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For use in the detection of phospholipids and related compounds.

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AMMC is demethylated by Human CYP2D6 to the fluorescent product 3-[2-(N,N-diethyl-N-methylammonium)ethyl]-hydroxy-4-methylcoumarin.

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Xylidyl blue I, (4-Hydroxy-3-[2-hydroxy-3-(2,4-dimethylphenylaminocarbonyl)-1-naphthylazobenzenesulfonic acid sodium salt; Magonsulfonate)

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Silver enhancer solution A has been used for silver staining.

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Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.

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Fast Red RC Salt, (2-Amino-4-chloroanisole diazotated zinc chloride complex; 5-Chloro-2-methoxybenzenediazonium chloride hemi(zinc chloride) salt; Diazo Fast Red RC)

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Direct Red 81, Dye content 50 % (DR 81; Disodium 7-benzamido-4-hydroxy-3-[[4-[(4-sulphonatophenyl)azo]phenyl]azo]naphthalene-2-sulphonate)

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Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg₂(NO₃)₂. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes colour from pale green to blue. Binding of TBPB to proteins is dependent on pH.

Supplier: SIGMA ALDRICH MICROSCOPY

When mixed with a 4% sodium nitrite solution, a freshly hexazotized pararosaniline solution is produced. May be used in a variety of cytochemistry procedures, including Sigma Procedures 181B and 181C.

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Intended for use in the Sigma α-Butyrate Esterase kit, procedure 181B

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Fluorescein isothiocyanate isomer I–Celite® has been conjugated with antibody for fluorescence imaging in cells and flow cytometry analysis.

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Bromophenol Blue is a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

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Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable as a laser dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Azobenzene 98%, Sigma-Aldrich®

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Amyloid protein is detected in tissue with Congo red, a metachromatic stain. Staining is intensified by pretreatment of tissue with alkaline sodium chloride.

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Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

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Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

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Azure A, Sigma-Aldrich®

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Safranine O, Sigma-Aldrich®

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Silver enhancer solution B has been used in the enhancement of metallic nanoparticles and silver staining.

Supplier: SIGMA ALDRICH MICROSCOPY

2, 5-Dibromo-6-isopropyl-3-methyl-1, 4-benzoquinone has been used as a metabolic inhibitor of violaxanthin (V) cycle of Pelvetia canaliculata and is used as a photosynthetic electron transport chain (pETC) inhibitor.

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Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

Supplier: SIGMA ALDRICH MICROSCOPY

Used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.