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377 Results for: "OMEGA BIO-TEK"

DNA markers, 1 kbp

Supplier: OMEGA BIO-TEK

The 1 kbp DNA marker is ideal for determining the size of double-stranded DNA within the range 1 kbp to 10 kbp. The ladder consists of eight fragments and is pre-mixed with loading dye and ready to use.

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E.Z.N.A.® Plasmid DNA Mini Kit I, (Q-spin, capless)

Supplier: OMEGA BIO-TEK

Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

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Mag-Bind® Fit24™ cfDNA Kit

Mag-Bind® Fit24™ cfDNA Kit

Supplier: OMEGA BIO-TEK

Pre-scripted solution for the purification of cfDNA from up to 4 ml plasma samples.

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DNA plates for genomic/plasmid DNA extraction, E-Z® 96

Supplier: OMEGA BIO-TEK

E-Z® 96 DNA plates are silica glass fibre plates can bind up to 50 μg of genomic DNA or 20 μg of plasmid DNA per prep. Each well has a capacity of 1 ml and the plates are semi skirted. E-Z 96 DNA plates can be processed in vacuum manifolds and/or by centrifugation.

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Mag-Bind® PX blood RNA 96 kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

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Deep well collection plates, 96-Well, 2,2 ml

Supplier: OMEGA BIO-TEK

96-well PP collection plates with 2,0 ml well volume for lysis, sample cultivation, and flow-through collection. This square well plate allows for larger volume than the equivalent round well plate in a standard microplate format.

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Mag-Bind® Universal Pathogen Core Kit

Supplier: OMEGA BIO-TEK

Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

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E.Z.N.A.® Endo-Free plasmid kits

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

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E.Z.N.A.® SP plant DNA kit

E.Z.N.A.® SP plant DNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® SP plant DNA kit is specially designed for the rapid and reliable isolation of high quality total cellular DNA from a variety of plant species. Up to 100 mg of fresh samples or 30 mg of dry samples can be processed in less than 40 minutes. Purified DNA is suitable for most downstream applications including PCR, restriction digestions and hybridisation techniques.

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E.Z.N.A.® blood RNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

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Tissue direct PCR kits

Supplier: OMEGA BIO-TEK

The tissue direct PCR kit is a buffer system for direct PCR from animal tissue and cultured cells that effectively lyses the tissue and neutralises inhibitors. The kit requires as little as 5 to 10 mg of tissue.

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Bacterial RNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

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E.Z.N.A.® Insect DNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Insect DNA kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridisation techniques.

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Food DNA kit, E.Z.N.A.®

Food DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Food DNA Kit allows rapid and reliable isolation of high quality DNA from complex matrixes such as processed food, chocolate, cereals and meat.

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Mag-Bind® Endo-free Plasmid Midi Kit

Mag-Bind® Endo-free Plasmid Midi Kit

Supplier: OMEGA BIO-TEK

Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

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E-Z 96® Plant DNA kit

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA kit allows rapid and reliable isolation of high quality total cellular DNA from a wide variety of plant species and tissues in a 96-well format. E-Z 96® Plant DNA kits utilise a buffer system adapted from the SP Plant DNA system, which is suitable for a variety of plants, such as those with unusually high levels of phenolic compounds or polysaccharides (for example, cotton, pine and peanut samples). Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation techniques.

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Genomic DNA isolation from blood, E.Z.N.A.® SQ blood DNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® SQ blood DNA kit provides a reliable method for the isolation of high molecular weight genomic DNA from fresh, frozen, or anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. There is no need for phenol/chloroform extractions, and time consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ blood DNA method is ready for downstream applications such as PCR, southern blotting and restriction enzyme digestion.

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Forensic DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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RNA isolation, total RNA kit, E.Z.N.A.® MicroElute

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

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E.Z.N.A.® total DNA/RNA isolation kit

E.Z.N.A.® total DNA/RNA isolation kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

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Mag-Bind® Fit24™ Blood and Tissue DNA Kit

Mag-Bind® Fit24™ Blood and Tissue DNA Kit

Supplier: OMEGA BIO-TEK

Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

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MicroElute® Cycle-Pure & Gel Extraction Kit, Omega Bio-tek

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

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Mag-Bind® cfDNA kit

Mag-Bind® cfDNA kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500 to 4000 µl plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or with automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

DNA wash buffer concentrate, low salt spin column was solution (makes 500 ml)

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Mag-Bind® Total RNA 96 kit

Supplier: OMEGA BIO-TEK

Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.

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Dye terminator removal, Mag-Bind® SeqDTR™

Dye terminator removal, Mag-Bind® SeqDTR™

Supplier: OMEGA BIO-TEK

Mag-Bind® SeqDTR™ is designed for the effective and reliable removal of unincorporated terminators from sequencing reaction. Sequencing products are first mixed with the Mag-Bind® SeqDTR™. DNA then selectively binds to the Mag-Bind® SeqDTR™ particles. With two rapid wash steps, trace contaminants such as nucleotides, primers and small, non targeted amplification products are removed.

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RNA isolation, total RNA kit, E-Z 96®

Supplier: OMEGA BIO-TEK

The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

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E.Z.N.A.® PX Blood RNA Kit

Supplier: OMEGA BIO-TEK

Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.

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Next generation sequencing (NGS), DNA/RNA clean up Mag-Bind® TotalPure

Next generation sequencing (NGS), DNA/RNA clean up Mag-Bind® TotalPure

Supplier: OMEGA BIO-TEK

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.

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