350 Results for: "Electrophoresis Buffers"

Supplier: VWR Collection

Designed for ease of use and safe operation, the shiroGEL horizontal electrophoresis systems and accessories are built to withstand the rigours of everyday use. For leakproof performance the gel boxes and gel trays are moulded from thick acrylic. UV transparent gel trays aid in visualisation of samples. Cassettes protect the electrodes and allow for easy replacement. Gel casting is simple with the durable rubber casting gates. Slots in the sides of the trays allow for easy comb placement. The two-piece design of the combs allows for vertical adjustment, giving the user control over the depth of the well. The combs supplied with the systems are 1,5 mm thick. Multiple combs, including those compatible with multichannel pipettes and different thicknesses, are also available. The 'EasyLift' gel box lid is easily removed using the side tabs and pressure pads. The lid is domed, to prevent condensation from dripping onto the gel, and is surrounded by a drip ring, to help recover the condensate and maintain buffer concentration.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: VWR Chemicals

EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

Supplier: VWR Collection

The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.

Supplier: Thermo Fisher Scientific

6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.

Supplier: Agilent

CEP coated capillaries contain a permanently bonded polymer coating. This CEP coating shields the silanol functionality of the capillary surface and helps prevent sample adsorption. Additionally, EOF is nearly eliminated, making the capillary ideal for applications such as DNA separations with sieving polymer buffers.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B2 EasyCast™ mini horizontal gel system provides flexibility in a small footprint and is available with or without buffer exchange ports. The Owl™ B3 with built-in recirculation system prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl™ B3 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs. As the recirculation system is built into the buffer chamber, no external pumps, tubing or stirring bars are required.

Supplier: LONZA

Formaldehyde sample buffer, For: FlashGel® system

Supplier: G-Biosciences

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.

Supplier: EDVOTEK

0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: Agilent

PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol. This coating minimises hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pHrange, even under basic conditions from 2,5 to 9,5. This stability allows the use of many common CE buffers. Because the silica surface is covered, many proteins and amines can be analysed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility may be improved.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ A2 horizontal gel system is a simple, convenient and fast system for detailed DNA/RNA analysis on multiple samples. It is available with or without buffer exchange ports. The Owl A5 large gel system with built-in recirculation prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl A5 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs.

Supplier: Serva

LAEMMLI ELECTROPHORESIS BUFFER, 10FOLD C 1 * 2 L

Supplier: Boster Bio

SDS-PAGE Electrophoresis Buffer 1 * 1 L

Supplier: Serva

LAEMMLI ELECTROPHORESIS BUFFER, 10FOLD C 1 * 10 L

Supplier: Serva

TRIS-TRICINE/SDS ELECTROPHORESIS BUFFER 1 * 1 L

Supplier: EDVOTEK

electrophoresis buffer 50x tae 1 * 1 items

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: G-Biosciences

molecular grade, concentrated running buffer for dna and rna electrophoresis. 1 * 250 mL

Supplier: G-Biosciences

lds sample loading buffer [4x] is used to prepare protein samples for sds polyacrylamide gel electrophoresis. 1 * 10 mL

Supplier: G-Biosciences

Kit Introduction to Agarose Electrophoresis includes DNA plasmid 1, DNA plasmid 1 (digested), DNA plasmid 2, DNA plasmid 2 (digested ), agarose, DNA stain (500X), DNA ladder (1kb), DNA loading buffer (6X), TAE buffer (50X), centrifuge tubes (2ml). 1 * 1 KIT

Supplier: EMBITEC

MiniLab a taste of genetics - Extraction, PCR and electrophoresis reagents. Includes DNA extraction solution, Taq polymerase, primers, restriction enzyme, GreenGel cups, running buffer and accessoires for ten groups of students. 1 * 1 KIT