348 Results for: "Electrophoresis Buffers"

Supplier: G-Biosciences

Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: MP Biomedicals

Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.

Supplier: Agilent

PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol. This coating minimises hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pHrange, even under basic conditions from 2,5 to 9,5. This stability allows the use of many common CE buffers. Because the silica surface is covered, many proteins and amines can be analysed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility may be improved.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Supplier: MP Biomedicals

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Store at Room Temperature (15-30 °C). Store dessicated.

Supplier: LONZA

FlashGel™ system for RNA is optimised for the unique requirements of RNA, and is an ideal tool for rapid analysis of sample integrity. High quality, intact RNA is essential for consistent results in gene expression, Northern analysis, cDNA library construction and cDNA labelling for microarrays. Most protocols recommend checking RNA prior to downstream analysis.

Supplier: MP Biomedicals

Borax is a salt of boric acid which is used as a buffer. It has a co-complexing ability with other agents in water to form complex ions with various substances. It is also commonly utilized in chromatography and electrophoresis studies.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: MP Biomedicals

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ A2 horizontal gel system is a simple, convenient and fast system for detailed DNA/RNA analysis on multiple samples. It is available with or without buffer exchange ports. The Owl A5 large gel system with built-in recirculation prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl A5 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: G-Biosciences

Kit Protein Folding Study includes PAGE mix (19:1 acrylamide/bisacrylamide), ammonium persulfate (APS), detergent solution (10% SDS) , PAGE stacking buffer, PAGE separating buffer, TEMED, sample loading buffer (2X concentrated), electrophoresis runni 1 * 1 KIT

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution. 200 mL of 50X buffer 1 * 200 mL

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution.500 mL of 50X buffer 1 * 500 mL

Supplier: Serva

LAEMMLI ELECTROPHORESIS BUFFER, 10FOLD C 1 * 10 L

Supplier: Serva

LAEMMLI ELECTROPHORESIS BUFFER, 10FOLD C 1 * 2 L

Supplier: Boster Bio

SDS-PAGE Electrophoresis Buffer 1 * 1 L

Supplier: Serva

TRIS-TRICINE/SDS ELECTROPHORESIS BUFFER 1 * 1 L

Supplier: EDVOTEK

electrophoresis buffer 50x tae 1 * 1 items

Supplier: EDVOTEK

This powdered blend Tris-Borate EDTA buffer (TBE), has been optimized for agarose gel electrophoresis of nucleic acids. Dissolve pow der in 500 ml of distilled or deionized water to prepare a 10x concentrate which can be diluted 10-fold to make a 1x 1 * 1 items

Supplier: G-Biosciences

molecular grade, concentrated running buffer for dna and rna electrophoresis. 1 * 250 mL

Supplier: G-Biosciences

Kit Introduction to Agarose Electrophoresis includes DNA plasmid 1, DNA plasmid 1 (digested), DNA plasmid 2, DNA plasmid 2 (digested ), agarose, DNA stain (500X), DNA ladder (1kb), DNA loading buffer (6X), TAE buffer (50X), centrifuge tubes (2ml). 1 * 1 KIT

Supplier: G-Biosciences

lds sample loading buffer [4x] is used to prepare protein samples for sds polyacrylamide gel electrophoresis. 1 * 10 mL

Supplier: EMBITEC

MiniLab a taste of genetics - Extraction, PCR and electrophoresis reagents. Includes DNA extraction solution, Taq polymerase, primers, restriction enzyme, GreenGel cups, running buffer and accessoires for ten groups of students. 1 * 1 KIT