12531 Results for: "ENZO LIFE SCIENCES"

Supplier: Enzo Life Sciences

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. 120S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. The hybridoma secreting the antibody to subunits HC2, HC3, HC8, HC9, Iota and Zeta was generated by fusion of spenocytes from Balb/c mice which had recieved repeated immunisation with dinitrophenylated proteasomes.

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: Enzo Life Sciences

Deconjugating Enzyme (DCE)

Supplier: Enzo Life Sciences

Cell permeable, potent, selective and ATP-competitive inhibitor of CDK2/cyclin A (IC50=22nM), CDK1/cyclin B (IC50=40nM) and CDK4/cyclin D1 (IC50=200nM). Has no effect on the activity of PKC, p38, PDGFRβ and EGFR (IC50>10µM). Displays anti-proliferative and pro-apoptotic properties in tumor cells. Inhibits proliferation of growth factor-stimulated colon carcinoma cells by binding to CDK2 and thus preventing the phosphorylation of pRb and its dissociation from E2F.

Supplier: Enzo Life Sciences

Competitive inhibitor of CDK1/cyclin B (IC50=5µM) and CDK2/cyclin A (IC50=12µM). Pharmacologically distinct from the relative specific inhibitor of CDK1/2 roscovitine.

Supplier: Enzo Life Sciences

CDK/cyclin inhibitor

Supplier: Enzo Life Sciences

gamma-Secretase inhibitor

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: Enzo Life Sciences

Sequence derived from human/mouse dishevelled-2 [Cys+600-614]. Used to generate the rabbit polyclonal antiserum to dishevelled-2 (BML-DA4270).

Supplier: Enzo Life Sciences

Immunization and blocking peptide for Glutamic Acid Decarboxylase (GAD) antibodies (BML-GC3008 and BML-GC3108).

Supplier: Enzo Life Sciences

Host: Rat, Isotype: IgG2a

Supplier: Enzo Life Sciences

Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 aa with molecular weight of 55kDa as a monomer and 110kDa as a disulfide-linked dimer. It acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG2a

Supplier: Enzo Life Sciences

CD66e (CEACAM5; CEA) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180'000-200'000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cells of the tongue, esophagus and cervix. CD66e is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).

Supplier: Enzo Life Sciences

For the quantitative determination of active human VEGF-C (vascular endothelial growth factor C) in cell culture supernatants, serum and other body fluids.

Supplier: Enzo Life Sciences

SOCS-1 also known as JAB and (STAT)-induced STAT inhibitor 1 (SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signalling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin. SOCS1/JAB is rapidly induced by LPS and negatively regulates LPS signalling.

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography.

Supplier: Enzo Life Sciences

A new class of proteasome inhibitors, considerably extended in comparison with the commonly used fluorescent substrates and peptide-based inhibitors, has been prepared. Evaluation of these new compounds revealed a set of highly potent proteasome inhibitors that target all individual active subunits with comparable affinity, unlike the other inhibitors described to date. These compounds are more potent in living cells than their shorter peptide vinyl sulphone counterparts. Modification of the most active compound, adamantaneacetyl-(6-aminohexanoyl)3-(leucyl)3-vinylmethyl-sulphone itself capable of proteasome inhibition in living cells, afforded a new set of radio- and affinity labels.

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG1

Supplier: Enzo Life Sciences

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack.The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Brefeldin A (Prod. No. BML-G405) causes the contents of the Golgi stack to be redistributed into the endoplasmatic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38, following Brefeldin A treatment, a useful tool for the discrimination of the TGN from the Golgi stack.

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG1

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG2b

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG3

Supplier: Enzo Life Sciences

AMPA/kainate receptor antagonist