324 Résultats pour : "EDVOTEK"

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This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

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Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.

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Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.

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Classroom molecular biology toys and games

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Single rack.

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Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.

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Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

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Bacterial plating agar (30 g)

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LB media, 100 g, Application: Selective media, Selective for: E.coli, Standards: -

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Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

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In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

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Our best-selling M12 Complete™ just got even better. The M12 Ultra™ includes the M12 Complete™ electrophoresis package and the all-new TruBlu™ Jr. Blue light transilluminator. This dynamic duo enables real-time visualisation of SYBR® Safe or other blue light-reactive dye agarose gels.

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This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

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This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.

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Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.

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Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

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Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.

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Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

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Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

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Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

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Basic concepts of DNA fingerprinting are featured in this Ready-to-Load™ kit by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have commited the crime.

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In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

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0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

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Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin

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The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

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For 20 gels.

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Each PCR Bead contains Taq DNA polymerase, Taq DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.

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Microorganism Tests, Escherichia coli, Species: Escherichia coli

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Practical, durable safety spectacles offering good protection and comfort.

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This Lab activity shows students what real chromosomal DNA Looks Like and allows them to explore the procedures involved in DNA extraction.