2131 Results for: "Cytiva"
Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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illustra™ ExoProStar™ 1-STEP kits
Supplier: Cytiva
illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.
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Affinity chromatography columns, HisTrap™ FF
Supplier: Cytiva
HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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Size exclusion chromatography columns, illustra™ NAP™
Supplier: Cytiva
NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.
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Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Affinity chromatography columns, HiTrap™ NHS-Activated HP
Supplier: Cytiva
HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.
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Ficoll-Paque® PLUS and Ficoll-Paque® PREMIUM Centrifugation Media, Cytiva
Supplier: Cytiva
Ficoll-Paque™ PREMIUM products are a range of sterile, ready to use density gradient media for the preparation of mononuclear cells.
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Sera-Mag carboxylate-modified magnetic beads and SpeedBeads
Supplier: Cytiva
Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH2).
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illustra™ ProbeQuant™ G-50 micro columns
Supplier: Cytiva
ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
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PCR beads, illustra™ PuReTaq Ready-To-Go™
Supplier: Cytiva
PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.
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Accessories for chromatography systems
Supplier: Cytiva
Flow restrictor, FR-902
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Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
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HiLoad Superdex columns, 200 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
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illustra™ MicroSpin™ G-25 columns
Supplier: Cytiva
For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.
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Small-scale SEC columns, Superose 6 Increase
Supplier: Cytiva
Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.
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Affinity chromatography columns, HisTrap™ excel
Supplier: Cytiva
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
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Anion exchange chromatography columns, HiTrap™ Q FF
Supplier: Cytiva
HiTrap Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow
Supplier: Cytiva
DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.
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Superdex 200 Increase small-scale SEC columns
Supplier: Cytiva
Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10000 to 600000.
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PEEK Tubing
Supplier: Cytiva
Polyetheretherketone tubing (PEEK) is intended for use with AKTA systems.
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Protein purification system, ÄKTA™ start
Supplier: Cytiva
ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.
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2D Clean-Up kit
Supplier: Cytiva
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
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Affinity chromatography columns, HisTrap™ FF crude
Supplier: Cytiva
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
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Affinity chromatography columns, HiTrap™ Heparin HP
Supplier: Cytiva
HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins.
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Disposable PD-10 desalting columns
Supplier: Cytiva
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
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Immobiline™ drystrip cover fluid
Supplier: Cytiva
Immobiline™ drystrip cover fluid
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Western blotting detection reagent, Amersham™ ECL™ Prime
Supplier: Cytiva
Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.